Bilayer of retinal pigmented epithelium and photoreceptors and use thereof
AssigneesFUJIFILM Cellular Dynamics, Inc., The United Sates of America, as Represented by the Secretary, Dept of Health and Human Services
InventorsAndrew DIAS, Erich BERNDT, Lucas CHASE, Juan AMARAL, Arvydas MAMINISHKIS, Kapil BHARTI
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Abstract
Provided herein are methods of producing a distinct bilayer culture of retinal epithelial cells (RPE) with photoreceptor cells and/or photoreceptor precursor cells (PR/PRP). Further provided herein is a therapy comprising transplantation of the RPE and PR/PRP bilayer as well as methods for testing candidate drugs using the bilayer.
Claims154
A tissue replacement implant comprising photoreceptor precursor cells (PRP) and/or photoreceptor cells (PR) in combination with retinal pigment epithelium cells (RPE) on a biodegradable scaffold.
The tissue replacement implant of any of claims 1-26, wherein the RPE are present at a density of about 300,000 cells/cm2 to about 800,000 cells/cm2 28 The tissue replacement implant of any of claims 1-27, wherein the PR/PRP are present at a density of about 100,000 cells/cm2 to about 10,000,000 cells/cm2.
A method for producing a PR/PRP-RPE bilayer comprising:
- (a) seeding RPE in a tissue culture medium in an upper compartment of a multi-well support with a tissue culture insert;
- (b) seeding PR/PRP in a tissue culture medium in the upper compartment of said multi-well support, directly in contact with RPE, wherein medium pressure of the lower compartment is higher than medium pressure of the higher compartment;
- and
- (c) culturing for a period of time sufficient to produce the PR/PRP-RPE bilayer.
The method of any of claims 86-118, wherein the PR/PRP are rod-predisposed.
A RPE-PR/PRP bilayer cell composition comprising a distinct bilayer of mature PRPs cultured on polarized RPE.
A method of treating an ocular injury or disorder in a subject comprising transplanting an effective amount of a retinal epithelial cells (RPE) and PR/PRP (RPE-PR/PRP) bilayer composition to an eye of the subject.
Dependencies are inferred from the claim text.