BW6 Specific CAR Designed To Protect Transplanted Tissue From Rejection
AssigneesThe Trustees of the University of Pennsylvania, One Lambda
InventorsJames L. Riley, Gavin Ellis, Jar-How Lee, Neng Jen Remi Shih, Thoa Nong
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Abstract
The present invention includes compositions and methods for an HLA-BW6 specific chimeric antigen receptor (CAR). In certain embodiments the HLA-BW6 specific CAR is expressed on a T regulatory cell. In certain embodiments, the HLA-BW6 specific CAR protects transplanted tissue from rejection.
Claims38
A modified immune cell or precursor cell thereof, comprising a chimeric antigen receptor (CAR) having affinity for HLA-BW6, wherein the CAR comprises an HLA-BW6 binding domain, a transmembrane domain, and an intracellular domain.
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The expression construct of claim 41, wherein the expression construct comprises an EF-1α promoter.
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An antibody or fragment thereof capable of binding HLA-BW6, wherein the antibody or fragment thereof comprises:
- a heavy chain variable region that comprises three heavy chain complementarity determining regions (HCDRs), wherein HCDR1 comprises the amino acid sequence of SEQ ID NO:
- 7, HCDR2 comprises the amino acid sequence of SEQ ID NO:
- 8, and HCDR3 comprises the amino acid sequence of SEQ ID NO:
- 9;
- and
- a light chain variable region that comprises three light chain complementarity determining regions (LCDRs), wherein LCDR1 comprises the amino acid sequence of SEQ ID NO:
- 10, LCDR2 comprises the amino acid sequence of SAK, and LCDR3 comprises the amino acid sequence of SEQ ID NO:
- 12.
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A nucleic acid comprising a polynucleotide sequence encoding the antibody or fragment thereof of claim 72.
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Dependencies are inferred from the claim text.