Rare earth element binding protein
OwnerBradley S. HEATER, Katarzyna H. KUCHARZYK +5inventors
InventorsBradley S. HEATER, Katarzyna H. KUCHARZYK, Daniel R. MARZOLF, Steven A. HIGGINS, Jonathan W. PICKING, Scott BANTA, Farid KHOURY
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Abstract
This invention relates to rare earth element binding protein and methods of recovering a rare earth element (REE) from a sample.
Claims22
A rare earth element (REE) binding protein comprising a repeating sequence X1X2X3X4X5X6X7X8X9, wherein X denotes any amino acid, and X1 is D, X2 is Y, T, or S, X3 is D or N, X4 is G or E, X5 is D or N, X6 is G, X7 is Y, Q or V, X8 is A, V, or Y, X9 is D.
A rare earth element (REE) binding protein wherein the REE binding protein comprises a sequence with at least 75% identity to SEQ ID. NO. 1.
A method of recovering a rare earth element (REE) from a sample comprising:
- a. introducing a REE binding protein to the sample wherein the REE binding protein comprises a repeating sequence X1X2X3X4X5X6X7X8X9 wherein X denotes any amino acid, and X1 is D, X2 is Y, T, or S, X3 is D or N, X4 is G or E, X5 is D or N, X6 is G, X7 is Y, Q or V, X8 is A, V, or Y, X9 is D;
- b. recovering the REE from the sample.
A method of recovering a rare earth element (REE) from a sample comprising:
- a. introducing a REE binding protein to the sample wherein the REE binding protein comprises a sequence with at least 75% identity to SEQ ID NO. 1;
- and
- b. recovering the REE from the sample.
The method of claim 16 wherein the REE binding protein indicates a difference in selectivity toward light REEs versus heavy REEs.
A method of recovering a rare earth element (REE) from a sample comprising:
- a. providing REE binding protein immobilized on a solid matrix where the REE binding protein comprises a repeating sequence X1X2X3X4X5X6X7X8X9 X denotes any amino acid, and X1 is D, X2 is Y, T, or S, X3 is D or N, X4 is G or E, X5 is D or N, X6 is G, X7 is Y, Q or V, X8 is A, V, or Y, X9 is D;
- b. introducing a sample containing one or more REEs onto said REE binding protein immobilized on said solid matrix;
- c. loading said one or more REEs onto said REE binding protein immobilized on said solid matrix;
- d. unloading said one or more REEs from said REE binding protein immobilized on said solid matrix.
The method of claim 20 wherein said unloading of said one or more REEs from said REE binding protein comprises adjusting pH.
A method of recovering a rare earth element (REE) from a sample comprising:
- a. providing REE binding protein immobilized on a solid matrix where the REE binding protein comprises a sequence with at least 75% identity to SEQ ID NO. 1
- b. introducing a sample containing one or more REEs onto said REE binding protein immobilized on said solid matrix;
- c. loading said one or more REEs onto said REE binding protein immobilized on said solid matrix;
- and
- d. unloading said one or more REEs from said REE binding protein immobilized on said solid matrix.
The method of claim 22 wherein said unloading of said one or more REEs from said REE binding protein comprises adjusting pH.
Dependencies are inferred from the claim text.