US 2026/0102491 A1 · METHOD FOR IN VITRO LARGE-SCALE EXPANSION OF DOUBLE-NEGATIVE T CELLS RICH IN TSCM AND TCM

Abstract

The present invention relates to a method for the in vitro large-scale expansion of double-negative T cells rich in Tscm and Tem. Specifically, the method comprises the step of: (a) providing an initial sample I of peripheral blood obtained from a donor, (b) pretreating the initial sample I to obtain sample II, and (c) culturing sample II in a culture system containing a culture medium suitable for the growth of DNT cell, thereby obtaining a sample III. The expansion process of the present invention is simple and stable, and can achieve the in vitro large-scale culturing of DNT cells rich in Tscm and Tcm cell characteristics. The purity of the final product DNT cells (CD3+CD4−CD8−) is ≥85%, which meets the requirements for clinical use.

Claims16

Dependent claims

An expansion method for double-negative T cells enriched with Tscm cells and Tcm in vitro, comprising steps:

(a) providing an initial sample I of peripheral blood obtained from a donor;
(b) pretreating the initial sample I to obtain sample II;
(c) culturing sample II in a culture system containing a culture medium suitable for the growth of DNT cell, thereby obtaining a sample III, wherein one or more cytokines selected from the following group are added to the culture system:
5-50 ng/ml recombinant human interleukin 21, 1-10 ng/ml recombinant human interleukin 1β, 5-50 ng/ml recombinant human interleukin 7, 5-50 ng/ml recombinant human interleukin 15, 5-50 ng/ml recombinant human interleukin 12;
wherein, in step (c), anti-human CD3 antibody is not added to the culture system.

2 more elements

Dependencies are inferred from the claim text.

Method for in vitro large-scale expansion of double-negative t cells rich in tscm and tcm

Life of this patent

Method for in vitro large-scale expansion of double-negative t cells rich in tscm and tcm

Life of this patent

Abstract

Claims16