Method for selecting an s. lycopersicum plant tolerant to high temperature conditions
AssigneeEnza Zaden Beheer B.V.
InventorsLouison Eliane Liard, Albert Grit, Nejra Solo, Cornelis Walter Verweij
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for selecting a S. lycopersicum plant that is tolerant to high temperature conditions and has an agronomical phenotype, wherein the plant is absent of an AP locus. The methods furthermore comprise the selecting a plant that is resistant to Tomato Brown Rugose Fruit Virus (TBRFV) comprising a TBRFV resistance gene. Furthermore, the invention relates to high temperature tolerant tomato plants (S. lycopersicum) that show an agronomical phenotype and in addition are preferably resistant to TBRFV.
Claims12
A method for selecting an S. lycopersicum plant that is tolerant to high temperature conditions, having an agronomical phenotype, wherein the method comprises the steps of;
- a) screening of S. lycopersicum plants for the presence of an AP locus on chr 9, wherein the presence of the AP locus is identified by the presence of one or more of an “T” on position 379858 in combination with a “C” on position 784380, preferably the presence of a “T” on position 428480 in combination with a “G” on position 601416, more preferably the presence of a “G” on position 474095 in combination with a “T” on position 571352,
- b) selecting the S. lycopersicum plants that do not comprise the AP locus,
- wherein said high temperature conditions is defined as;
- keeping said plant at a temperature of least 28° C. for at least 7 days, preferably for at least 14 days, preferably at least 30° C. at night for about 5 to 7 h, and at least above 36° C. at daytime for about 17 to 19 h,
- wherein the method in addition provides a S. lycopersicum plant that is resistant to TBRFV, wherein the method further comprises the step of screening and selecting a plant that is resistant to Tobamovirus, wherein said selection comprises establishing the presence of a TBRFV resistance gene comprising a coding sequence having at least 90% nucleotide sequence identity with SEQ ID No. 1.
An S. lycopersicum plant that is tolerant to high temperature conditions, wherein the plant is absent of an AP locus on chr 9 in its genome, wherein presence of the AP locus is identified by the presence of one or more of an “T” on position 379858 in combination with a “C” on position 784380, preferably the presence of a “T” on position 428480 in combination with a “G” on position 601416, more preferably the presence of a “G” on position 474095 in combination with a “T” on position 571352.
Dependencies are inferred from the claim text.