Tandem-trapped ion mobility spectrometry with microarray and biosensor
AssigneeThe Florida State University Research Foundation, Inc.
InventorsFanny Caroline Liu, Christian Bleiholder
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Abstract
Disclosed is an analytical platform for profiling and functional analysis of proteomes, which can unveil new biological insights potentially useful for instigating novel therapeutic interventions. The disclosed analytical platform for proteome profiling is constructed by integrating plasmonic biosensor microarrays with tandem-ion mobility spectrometry. The disclosed analytical platform can separate, identify, and characterize highly similar proteoforms as well as low abundance proteoforms from plasma or other samples.
Claims22
A method for analyzing ions comprising:
- providing ions from an ion source to a first trapped-ion mobility spectrometry analyzer, wherein the ion source is a peptide or RNA microarray;
- separating the ions according to mobility in the first trapped ion mobility spectrometry analyzer;
- selecting precursor ions of at least one limited mobility range;
- fragmenting the selected precursor ions into fragment ions;
- trapping the fragment ions in a second trapped ion mobility spectrometry analyzer;
- separating the fragment ions according to mobility in the second trapped ion mobility spectrometry analyzer;
- and
- detecting the separated fragment ions.
A method for analyzing ions comprising:
- providing ions from an ion source to a first trapped-ion mobility spectrometry analyzer, wherein the ion source is a peptide or RNA microarray;
- separating the ions according to mobility in the first trapped-ion mobility spectrometry analyzer;
- selecting ions of a limited mobility range;
- activating or reacting the selected ions;
- trapping the activated or reacted ions in a second trapped-ion mobility spectrometry analyzer;
- separating the trapped ions according to mobility in the second trapped-ion mobility spectrometry analyzer;
- and
- detecting the separated ions.
19-25. (canceled)
An ion mobility spectrometer comprising an peptide or RNA microarray as an ion source, a first trapped-ion mobility spectrometry analyzer, a second trapped-ion mobility spectrometry analyzer located downstream of the first trapped-ion mobility spectrometry analyzer, a fragmentation cell or reaction cell, which is located between the first and the second trapped-ion mobility spectrometry analyzer or is part of the second trapped-ion mobility spectrometry analyzer, and an ion detector.
28-31. (canceled)
Dependencies are inferred from the claim text.