Composition to improve salt tolerance in a plant or algae and methods of production thereof
AssigneeAssociaçãO Biopolis
InventorsPedro Humberto ARAÚJO RAMOS FERNANDES CASTRO, Leandro Miguel BASTOS PIRES, Herlander Anselmo QUEIRÓS PEREIRA AZEVEDO, Tiago Jorge MOREIRA LOURENÇO
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Abstract
The present disclosure relates to the use of a composition for improving the tolerance of a plant or algae to salt, comprising a molecule, wherein the molecule inhibits an expression of a specific nucleotide sequence. Furthermore, the present disclosure relates to a method and kit to determining the tolerance of a plant or algae to salt.
Claims36
A method for improving the tolerance of a plant or algae to salt, relative to a wild-type, comprising:
- introducing a composition into one or more plant or algae cells, wherein the composition comprises a molecule that reduces the expression in the plant or algae cells of a nucleotide sequence encoding an amino acid sequence comprising at least a sequence 8590% identical to the sequences of the following list SEQ ID No 69, SEQ ID No 70, SEQ ID No 71, SEQ ID No 72, SEQ ID No 73, SEQ ID No 74, SEQ ID No 75, SEQ ID No 76, SEQ ID No 77, SEQ ID No 78, SEQ ID No 79, SEQ ID No 80, SEQ ID No 81, SEQ ID No 82, SEQ ID No 83, SEQ ID No 84, and wherein the molecule is a protein molecule, a nucleic acid molecule or combinations thereof;
- and
- regenerating one or more plants or algae from said one or more plant or algae cells, wherein the regenerated plants or algae have increased salt tolerance relative to a wild-type plant or algae.
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A method for producing a genetically modified plant or algae with increased salt tolerance relative to a wild-type plant, the method comprising the following steps:
- introducing at least one mutation or exogenous nucleic acid into the genome of one or more plant or algae cells which results in reduced activity associated with a protein, wherein the protein comprising at least a sequence 90% identical to the sequences of the following list SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, SEQ ID No 29, SEQ ID No 30, SEQ ID No 31, SEQ ID No 32, SEQ ID No 33, SEQ ID No 34, SEQ ID No 35, SEQ ID No 36, SEQ ID No 37, SEQ ID 38, SEQ ID No 39, SEQ ID No 40, SEQ ID No 41, SEQ ID No 42, SEQ ID No 43, SEQ ID No 44, SEQ ID No 45, SEQ ID No 46, SEQ ID No 47, SEQ ID No 48, SEQ ID No 49, SEQ ID No 50, SEQ ID No 51, SEQ ID No 52, SEQ ID No 53, SEQ ID No 54, SEQ ID No 55, SEQ ID No 56, SEQ ID No 57, SEQ ID No 58, SEQ ID No 59, SEQ ID No 60, SEQ ID No 61, SEQ ID No 62, SEQ ID No 63, SEQ ID No 64, SEQ ID No 65, SEQ ID No 66, SEQ ID No 67, or a homologue thereof in said one or more plant or algae cells;
- regenerating one or more plants or algae from said one or more plant or algae cells;
- and
- selecting one or more plants or algae that have increased salt tolerance relative to a wild-type plant or algae.
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A method for screening a plant or algae with increased salt tolerance relative to a wild-type plant, the method comprising analyzing DNA of the plant or algae for the presence of at least one allele of a nucleotide sequence encoding a protein comprising at least a sequence 90% identical to the sequences of the following list SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, SEQ ID No 29, SEQ ID No 30, SEQ ID No 31, SEQ ID No 32, SEQ ID No 33, SEQ ID No 34, SEQ ID No 35, SEQ ID No 36, SEQ ID No 37, SEQ ID 38, SEQ ID No 39, SEQ ID No 40, SEQ ID No 41, SEQ ID No 42, SEQ ID No 43, SEQ ID No 44, SEQ ID No 45, SEQ ID No 46, SEQ ID No 47, SEQ ID No 48, SEQ ID No 49, SEQ ID No 50, SEQ ID No 51, SEQ ID No 52, SEQ ID No 53, SEQ ID No 54, SEQ ID No 55, SEQ ID No 56, SEQ ID No 57, SEQ ID No 58, SEQ ID No 59, SEQ ID No 60, SEQ ID No 61, SEQ ID No 62, SEQ ID No 63, SEQ ID No 64, SEQ ID No 65, SEQ ID No 66, SEQ ID No 67 or a homologue thereof, using at least one nucleic acid molecule suitable as a probe or primer which is capable of hybridizing to a gene comprising at least a sequence 90% identical to the sequences of the following list SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, or a homologue thereof.
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Dependencies are inferred from the claim text.