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US 2026/0098274 A1Published application
Root-mediated uptake of guide rna for genomic editing of a plant
AssigneeInari Agriculture Technology, Inc.
InventorsAran MCCAY, Michael Lee NUCCIO, Palak KATHIRIA
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Abstract
The present disclosure provides methods for editing a genomic target in a meristem of a plant that expresses Cas editing reagents by delivering to a plant root a guide RNA for the Cas nuclease. Various methods of delivering the guide RNA are provided. Plants edited using the methods are also provided.
Claims47
A method of editing a genomic target in a plant meristem comprising
- delivering a guide RNA for a Cas nuclease to a plant root, wherein the guide RNA is fused to a meristem transport segment (MTS),
- wherein the plant comprises nucleic acid encoding the Cas nuclease, and
- wherein a genomic target within a cell in the meristem is edited.
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Dependencies are inferred from the claim text.