Ultra-high molecular weight xanthan gum and its production strain and detection molecular marker

Abstract

An engineered strain that produces a xanthan gum is constructed by genetic engineering methods. Upon testing, it is found that the monosaccharide composition and repeating units of the xanthan gum are the same as those of an ordinary xanthan gum, but a molecular weight of the xanthan gum is greater than 2.0×107 Da, which is higher than a molecular weight range (0.2×107-2.0×107 Da) of the ordinary xanthan gum reported in the literature. Therefore, the xanthan gum is considered an ultra-high molecular weight xanthan gum, expanding application fields of the xanthan gum. A molecular marker for detecting a producing strain or processed products of the ultra-high molecular weight xanthan gum can rapidly identify an engineered strain synthesizing the ultra-high molecular weight xanthan gum and their mildly processed products.

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