# US 2026/0097084 A1 · PHARMACEUTICAL COMPOSITION CONTAINING CBFbeta-EXPRESSING VASCULAR SMOOTH MUSCLE CELLS OR CULTURE FLUID THEREOF AS ACTIVE INGREDIENT FOR PREVENTION OR TREATMENT OF AGE-RELATED DISEASES

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- Number: US 2026/0097084 A1
- Document ID: US20260097084A1
- Status: Published application
- Kind: A1
- Application number: 19/114,149
- Filed: 2023-09-18
- Published: 2026-04-09
- Earliest claimed: 2022-09-21
- Assignees: Kyungpook National University Industry-Academic Cooperation Foundation
- Applicants: Kyungpook National University Industry-Academic Cooperation Foundation
- Inventors: Je-Yong CHOI; Xiangguo CHE; Xian JIN
- Web page: https://patentgrep.com/patent/US20260097084A1

## CPC

- A61K 35/44: Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
- A61K 38/1709: {from mammals}
- A61K 45/00: Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61P 9/00: Drugs for disorders of the cardiovascular system
- A61P 19/10: for osteoporosis
- C12N 5/06: {Animal cells or tissues; Human cells or tissues (preservation of excised living parts A01N1/10)}
- C12N 15/85: for animal cells
- C12Q 1/6883: for diseases caused by alterations of genetic material
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a pharmaceutical composition containing Cbfβ (Core-binding factor subunit beta) expressing vascular smooth muscle cells or a culture fluid thereof as an active ingredient for the prevention or treatment of age-related diseases. It has been found that inhibition of Cbfβ expression in vascular smooth muscle cells promotes vascular calcification and osteoclast differentiation and that the culture fluid of Cbfβ-expressing vascular smooth muscle cells inhibits osteoclast differentiation. Thus, the composition can be advantageously utilized as a pharmaceutical composition for the prevention, treatment, alleviation, or diagnosis of age-related diseases.

## Claims

### Claim 1

A method for preventing or treating a geriatric disease, comprising administering a pharmaceutical composition comprising a pharmaceutically effective amount of a core-binding factor subunit beta (Cbfβ)-expressing vascular smooth muscle cell or a culture fluid thereof as an active ingredient to a subject in need of preventing or treating the geriatric disease.

### Claim 2 (depends on 1)

The method of claim 1, wherein the Cbfβ-expressing vascular smooth muscle cell or culture fluid thereof suppresses blood pressure increase; or osteoclast differentiation.

### Claim 3 (depends on 1)

The method of claim 1, wherein the geriatric disease is a vascular aging disease or a bone disease.

### Claim 4 (depends on 3)

The method of claim 3, wherein the vascular aging disease is one or more selected from the group consisting of vascular calcification, osteoporosis, osteoarthritis, arteriosclerosis, fatty liver, liver fibrosis, diabetes, and dementia.

### Claim 5 (depends on 3)

The method of claim 3, wherein the bone disease is one or more selected from the group consisting of fracture, osteoarthritis, rheumatoid arthritis, osteoporosis, and osteomalacia.

### Claim 6

A method for preventing or ameliorating a geriatric disease, comprising administering a health functional composition comprising a therapeutically effective amount of a core-binding factor subunit beta (Cbfβ)-expressing vascular smooth muscle cell or a culture fluid thereof as an active ingredient to a subject in need of preventing or treating the geriatric disease.

### Claim 7

A method for preventing or treating a geriatric disease, comprising administering a pharmaceutically effective amount of a pharmaceutical composition comprising an agent which induces expression or activity of a core-binding factor subunit beta (Cbfβ) protein or a gene encoding the same as an active ingredient to a subject in need of preventing or treating the geriatric disease.

### Claim at position 8

8-18. (canceled)