System and method for identifying an abnormal perfusion pattern

A computer implemented method for detecting one or more areas having an abnormal perfusion pattern in tissue of a subject during a medical procedure, the method comprising:

• continuously acquiring fluorescence images of the tissue, wherein the fluorescence images are associated with a fluorescent output signal correlated with an input signal defined by a series of boluses of at least one fluorescent imaging agent, and wherein the series of boluses is administered with a predefined and controlled duration between subsequent boluses, analyzing the fluorescence images,
• obtaining, defining and/or determining a normal perfusion pattern, at least in an intensity domain and/or in a time domain, and
• detecting, in the fluorescence images, possible tissue areas with an abnormal perfusion pattern based on the normal perfusion pattern and the fluorescent output signal.

A computer implemented method for establishing a time domain perfusion reference of a subject, comprising the steps of:

• continuously measuring a fluorescent output signal correlated with an input signal defined by a series of boluses of said fluorescent imaging agent, and wherein the series of boluses is administered with a predefined and/or controlled duration between subsequent boluses, and defining the subject specific time domain perfusion reference as the fluorescent output signal vs. time.