Triticale cultivar ns16t0220

A method of vegetatively propagating a plant of triticale cultivar NS16T0220, the method comprising:

• a. collecting tissue capable of being propagated from a plant of triticale cultivar NS16T0220, wherein a sample of seed of the cultivar was deposited under NCMA Accession No. 202409003;
• b. cultivating the tissue to obtain proliferated shoots to obtain rooted plantlets;
• and
• c. optionally growing plants from the rooted plantlets.

A method of introducing a desired trait into triticale cultivar NS16T0220 wherein the method comprises:

• a. crossing a NS16T0220 plant, wherein a representative sample of seed was deposited under NCMA Accession No. 202409003, with a plant of another triticale cultivar that comprises a desired trait to produce an F1 progeny plant;
• b. selecting one or more F1 progeny plants that have the desired trait to produce selected progeny plants;
• c. crossing the selected progeny plants with the NS16T0220 plants to produce backcross progeny plants;
• d. selecting for backcross progeny plants that have the desired trait and otherwise all of the physiological and morphological characteristics of triticale cultivar NS16T0220 to produce selected backcross progeny plants;
• and
• e. repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and otherwise all of the physiological and morphological characteristics of triticale cultivar NS16T0220.

A method of comparing and/or characterizing the genotype of a plant of triticale cultivar NS16T0220, a sample of seed of which has been deposited under NCMA Accession No. 202409003, comprising:

• a. obtaining a sample of nucleic acids from the plant of triticale cultivar NS16T0220.
• b. obtaining a sample of nucleic acids from a plant of a reference triticale cultivar;
• c. comparing the nucleic acids obtained from the plant of triticale cultivar NS16T0220 to the sample of nucleic acids obtained from the reference plant;
• and
• d. detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms is indicative of triticale cultivar NS16T0220 and/or gives rise to the expression of any one or more, or all, of the physiological and morphological characteristics of triticale cultivar NS16T0220.