Cell culture substrate equipped with layered myocardial cell sheet and method for manufacturing same, and method for evaluating normal myocardial cell sheet for transplantation

Abstract

An object of the present invention is to provide a cell culture substrate with laminated cardiomyocyte sheets for evaluating a curative effect by biological transplantation of a normal cardiomyocyte sheet and a method for producing the same. A further object of the present invention is to provide a method for evaluating a curative effect of a normal cardiomyocyte sheet for biological transplantation. The cell culture substrate with laminated cardiomyocyte sheets of the present invention includes two or more layers of cardiomyocyte sheets on a cell culture substrate, wherein the cell culture substrate includes an alternating arrangement portion in which a strip-shaped cell adhesion region and a strip-shaped cell adhesion suppression region are alternately arranged, the cell culture substrate with laminated cardiomyocyte sheets includes the two or more layers of cardiomyocyte sheets on at least the alternating arrangement portion of the cell culture substrate; and the cell culture substrate with laminated cardiomyocyte sheets includes, as the cardiomyocyte sheets, at least one layer of each of a disease cardiomyocyte sheet containing a differentiation-induced cardiomyocyte derived from a disease patient and a normal cardiomyocyte sheet containing a differentiation-induced cardiomyocyte derived from a healthy human.

Dependencies are inferred from the claim text.