Fluorescence-basedtoxin detection apparatus and method

A fluorescence-based method for detection and analysis of a toxin in a liquid sample, the method comprising:

• combining a diluted antibody mixture and the liquid sample to produce a preparation solution;
• combining a fluorescent compound absorbed onto colloidal graphene and the preparation solution to produce an analysis solution;
• shining a light onto the analysis solution and one or more reference solutions each having a different one of a plurality of known levels of toxin concentration of the toxin;
• observing a fluorescence intensity of the analysis solution from exposure to the light to detect whether the toxin is present in the analysis solution;
• and
• comparing the fluorescence intensity of the analysis solution from exposure to the light with one or more fluorescence intensities of the one or more reference solutions from exposure to the light to analyze a level of the toxin that is present in the analysis solution.