Method for barley hybrid seed production
AssigneeKWS SAAT SE & Co. KGaA
InventorsParastoo HOSEINZADEH, Stephanie JAHNKE, Volker MARWEDE, Jason WENZIG
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Abstract
The present invention relates to hybrid barley seed production and marker assisted selection of barley plants in order to obtain barley plants or seeds comprising an Ant 1 and/or Ant2 gene allowing color sorting of seeds.
Claims15
A method for identifying and/or selecting a barley plant or plant part, comprising screening for the presence of a polynucleotide comprising one or more, preferably all, molecular marker allele selected from Ant1_HapM1, Ant1_HapM2, Ant1_HapM3, Ant1_HapM4, Ant1_HapM5, and Ant1_HapM6 in the genome of the plant or plant part, preferably wherein said molecular marker allele is homozygous.
A method for identifying and/or selecting a barley plant or plant part, comprising screening for the presence of a polynucleotide comprising one or more molecular marker allele selected from Ant2_M1, Ant2_M2, Ant2_M3, Ant2_M4, Ant2_M5, Ant2_M6, Ant2_M7, Ant2_HapM1, Ant2_HapM2, Ant2_HapM3, Ant2_HapM4, Ant2_HapM5, Ant2_HapM6, and Ant2_HapM7, preferably selected from Ant2_M2, Ant2_M3, Ant2_M4, Ant2_M5, Ant2_M6, and Ant2_M7, or selected from Ant2_HapM1 combined with any one or more of Ant2_HapM2, Ant2_HapM3, Ant2_HapM4, Ant2_HapM5, Ant2_HapM6, or Ant2_HapM7 in the genome of the plant or plant part.
A male sterile barley plant or plant part, preferably a cytoplasmic male sterile barley plant or plant part, comprising one or more, preferably all, homozygous molecular marker allele selected from Ant1_HapM1, Ant1_HapM2, Ant1_HapM3, Ant1_HapM4, Ant1_HapM5, and Ant1_HapM6 in the genome of the plant or plant part, and comprising one or more of a homozygous or heterozygous molecular marker allele selected from Ant2_M1, Ant2_M2, Ant2_M3, Ant2_M4, Ant2_M5, Ant2_M6, Ant2_M7, Ant2_HapM1, Ant2_HapM2, Ant2_HapM3, Ant2_HapM4, Ant2_HapM5, Ant2_HapM6, and Ant2_HapM7, preferably selected from Ant2_M2, Ant2_M3, Ant2_M4, Ant2_M5, Ant2_M6, and Ant2_M7, or selected from Ant2_HapM1 combined with any one or more of Ant2_HapM2, Ant2_HapM3, Ant2_HapM4, Ant2_HapM5, Ant2_HapM6, or Ant2_HapM7.
An isolated polynucleotide having a sequence comprising, comprised in, or consisting of a sequence as set forth in any of SEQ ID NO:
- 1 to 32, or a unique fragment thereof, or the complement or reverse complement of any thereof.
Dependencies are inferred from the claim text.