# US 2026/0092273 A1 · Methods and control compositions for sequencing

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- Number: US 2026/0092273 A1
- Document ID: US20260092273A1
- Status: Published application
- Kind: A1
- Application number: 19/213,417
- Filed: 2025-05-20
- Published: 2026-04-02
- Earliest claimed: 2018-05-21
- Assignees: Battelle Memorial Institute
- Applicants: Battelle Memorial Institute
- Inventors: Rachel R. SPURBECK; Richard Mon Che CHOU; Anthony D. DUONG
- Web page: https://patentgrep.com/patent/US20260092273A1

## CPC

- C12N 15/1089: {Design, preparation, screening or analysis of libraries using computer algorithms}
- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- C12Q 1/6869: Methods for sequencing
- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 2563/185: Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
- C12Q 2600/166: Oligonucleotides used as internal standards, controls or normalisation probes
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to control compositions for sequencing and for chemical analyses, such as analytical chemistry analyses. More particularly, the invention relates to control compositions for sequencing and for chemical analyses having at least one barcode sequence fragment and at least one universal sequence fragment, and to methods of their use.

## Claims

### Claim 1

A sequencing control composition, said control composition comprising a nucleic acid construct comprising at least one barcode sequence fragment linked at its 5′ or 3′ end to at least one universal sequence fragment.

### Claim 2 (depends on 1)

The control composition of claim 1, wherein the control composition is configured for determining if cross-contamination between samples for sequencing or sample swapping has occurred.

### Claim 3 (depends on 1)

The control composition of claim 1, wherein the nucleic acid construct is a deoxyribonucleic acid construct.

### Claim 4 (depends on 1)

The control composition of claim 1, wherein the nucleic acid construct comprises at least a first universal sequence fragment and a second universal sequence fragment.

### Claim 5 (depends on 4)

The control composition of claim 4, wherein the first universal sequence fragment is linked to the 5′ end of the barcode sequence fragment, and wherein the second universal sequence fragment is linked to the 3′ end of the barcode sequence fragment.

### Claim 6 (depends on 5)

The control composition of claim 5, wherein the nucleic acid construct further comprises at least a first primer binding site fragment and a second primer binding site fragment, wherein the first primer binding site fragment is linked at its 3′ end to 5′ end of the first universal sequence fragment, and wherein the second primer binding site fragment is linked at its 5′ end to the 3′ end of the second universal sequence fragment.

### Claim 7 (depends on 6)

The control composition of claim 6, wherein the primer binding site fragments range in length from about 15 base pairs to about 30 base pairs.

### Claim 8 (depends on 6)

The control composition of claim 6, wherein the nucleic acid construct ranges in length from about 80 base pairs to about 300 base pairs.

### Claim 9 (depends on 1)

The control composition of claim 1, wherein the sequencing is whole genome sequencing.

### Claim 10 (depends on 6)

The control composition of claim 6, wherein the sequencing is amplicon sequencing.

### Claim 11 (depends on 1)

The control composition of claim 1, wherein the sequencing is Next Generation Sequencing.

### Claim 12 (depends on 1)

The control composition of claim 1, wherein the nucleic acid construct is encapsulated.

### Claim 13 (depends on 12)

The control composition of claim 12, wherein the nucleic acid construct is encapsulated in a liposome.

### Claim 14 (depends on 13)

The control composition of claim 13, wherein the liposome comprises a lipid selected from the group consisting of cholesterol, a lipopolysaccharide, a peptidoglycan, a PEG, a teichoic acid, a phospholipid, and combinations thereof.

### Claim 15 (depends on 1)

The control composition of claim 1, wherein the nucleic acid construct is incorporated into the genome of a microorganism.

### Claim 16 (depends on 1)

The control composition of claim 1, wherein the barcode sequence fragment comprises a unique sequence not present in any known genome.

### Claim 17 (depends on 1)

The control composition of claim 1, wherein the nucleic acid construct is incorporated into a plasmid.

### Claim 18 (depends on 6)

A kit comprising the control composition of claim 6.

### Claim 19 (depends on 18)

The kit of claim 18, further comprising a reagent selected from the group consisting of a reagent for nucleic acid extraction, a reagent for nucleic acid purification, a reagent for library preparation, and a reagent for sequencing.

### Claim 20 (depends on 18)

The kit of claim 18, further comprising a probe.