Novel process for purifying heparan-n-sulfatase
AssigneeThe Green Cross Corporation
InventorsKwanyoung KO, Dasom KIM, Sulgi YOO, Geunhye YEO, Minjung KIM, Dong Kyu JIN
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Abstract
The present invention is related to a method for purifying heparan-N-sulfatase from a heparan-N-sulfatase-containing solution including at least one impurity, the method comprising performing multi-mode chromatography (MMC) to obtain an eluate; and performing caprylate precipitation to obtain a supernatant. The method according to the present invention is capable of very efficiently removing HCP (host cell proteins) and of greatly improving the purity and stability of purified heparan-N-sulfatase.
Claims13
A method for purifying heparan-N-sulfatase from a heparan-N-sulfatase-containing solution including at least one impurity, the method comprising:
- performing multi-mode chromatography (MMC) to obtain an eluate;
- and
- performing caprylate precipitation to obtain a supernatant.
Dependencies are inferred from the claim text.