Simultaneous production of structural proteins from heterologous bacteriophage in cell-free expression system
AssigneesElisabeth FALGENHAUER, Technische Universität München
InventorsFriedrich SIMMEL, Kilian VOGELE, Sophie VON SCHÖNBERG
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Abstract
The present invention relates to multi-peptide structures comprising at least one heterogenous functional site wherein the at least one heterogenous functional site is composed of at least two homologous peptides, which differ by at least one amino acid, a method for providing such multi-peptide structures, compositions comprising such multi-peptide structures as well as the use of such multi-peptide structures and compositions as an universal anti-microbial agent, in particular in medicine, chemistry, biotechnology, agriculture and/or food industry.
Claims15
A multi-peptide structure comprising at least one heterogenous functional site wherein the at least one heterogenous functional site is composed of at least two homologous peptides, which differ by at least one amino acid.
Method, in particular in vitro method, for providing multi-peptide structures, in particular synthetic bacteriophages, comprising the steps of
- (a) providing an expression system, in particular a cell-free expression system,
- (b) adding nucleic acids encoding homologous bacteriophage proteins, preferably derived from different bacteriophages, in particular homologous bacteriophage tail fiber proteins and/or homologous bacteriophage capsid proteins, to the expression system,
- (c) expressing the nucleic acids encoding the homologous proteins, in particular homologous phage tail fiber proteins and/or homologous phage capsid proteins,
- (d) assembling of the expressed homologous proteins, in particular homologous phage tail fiber proteins and/or homologous phage capsid proteins, to provide the assembled multi-peptide structures, in particular the synthetic bacteriophages, and optional
- (e) isolating of the assembled multi-peptide structures, in particular the synthetic bacteriophages.
Dependencies are inferred from the claim text.