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US 2026/0092086 A1Published application
Antimicrobial lasso peptides
AssigneeThe Trustees of Princeton University
InventorsA. James LINK, Drew CARSON
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Abstract
Cloacaenodin is a lasso peptide resistant to cleavage by proteases and with antimicrobial properties, for example, with the ability to inhibit he growth of and kill Enterobacter species of bacteria.
Claims124
A method for inhibiting growth of a microorganism, comprising
- providing a cloacaenodin-class lasso peptide that is purified;
- and
- exposing the microorganism to the cloacaenodin-class lasso peptide,
- so that the growth of the microorganism is inhibited,
- wherein the cloacaenodin-class lasso peptide comprises a ring, a loop region, and a tail region,
- wherein the ring is bonded to the loop region,
- wherein the loop region is bonded to the tail region,
- wherein the cloacaenodin-class lasso peptide comprises a peptide sequence
- [SEQ ID NO. 1]
- GHSVDRIPEYFGPPGLPGPVLFYS,
- wherein 0, 1, or 2 of residues 2 through 8 of the peptide sequence are removed or replaced with another residue,
- wherein 0 or 1 residue is inserted after one of residues 1 through 8,
- wherein 0, 1, 2, 3, 4, or 5 of residues 12 through 24 of the peptide sequence are removed or replaced with another residue, and
- wherein 0, 1, 2, 3, or 4 residues are inserted after at least one of residues 11 through 21.
11 more elements
The method according to claim, wherein residue 8 of the peptide sequence is replaced by alanine (A).
A purified cloacaenodin-class lasso peptide, comprising
- a peptide sequence GHSVDRIPEYFGPPGLPGPVLFYS [SEQ ID NO. 1],
- wherein the peptide sequence comprises a ring, a loop region, and a tail region,
- wherein the ring is bonded to the loop region,
- wherein the loop region is bonded to the tail region,
- wherein 0, 1, or 2 of residues 2 through 8 of the peptide sequence are removed or replaced with another residue,
- wherein 0 or 1 residue is inserted after one of residues 1 through 8,
- wherein 0, 1, 2, 3, 4, or 5 of residues 12 through 24 of the peptide sequence are removed or replaced, and
- wherein 0, 1, 2, 3, or 4 residues are inserted after residues 11 through 21.
5 more elements
A method of producing a cloacaenodin-class lasso peptide, comprising
- refactoring the precursor, protease, cyclase, and exporter genes for cloacaenodin into a plasmid in vitro,
- transforming the plasmid into cells in vitro,
- growing the cells in vitro,
- inducing expression of the cloacaenodin-class lasso peptide by the cells in vitro,
- separating the cells and a supernatant, and
- obtaining purified cloacaenodin-class lasso peptide from the supernatant.
3 more elements
Dependencies are inferred from the claim text.