Systems and methods for delivering reagents to a flow cell
Assignee10x Genomics, Inc.
InventorsSarah Chan, Martin Sauzade, Yiran Zhang, Jay Kishore Nagin
Life of this patent
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Abstract
A system for delivering a plurality of reagents into a closed flow cell includes one or more reservoirs comprising reagents, a pump configured to cause a flow of the plurality of reagents, an aspiration device coupled to a first fluid channel, a closed flow cell having an inlet and an outlet, where the inlet is coupled to an end of the first fluid channel, and a de-bubbler coupled to the first fluid channel upstream of the closed flow cell. The de-bubbler is configured to divert one or more bubbles from the first fluid channel.
Claims35
A method comprising:
- providing a plurality of reservoirs, each reservoir of the plurality of reservoirs containing a reagent;
- using an aspiration device to aspirate a first reagent from a first reservoir of the plurality of reservoirs;
- flowing the first reagent from the aspiration device through a first fluid channel;
- delivering at least a portion of the first reagent from an end of the first fluid channel into an inlet of a closed flow cell;
- using the aspiration device to aspirate at least one bubble into the first fluid channel, the bubble upstream of the first liquid reagent;
- using the aspiration device to aspirate a second reagent from a second reservoir of the plurality of reservoirs;
- flowing the second reagent from the aspiration device through the first fluid channel, the second liquid reagent upstream of the at least one bubble, wherein the at least one bubble separates the first liquid reagent and the second liquid reagent in the first fluid channel;
- diverting the at least one bubble from the first fluid channel;
- delivering the second reagent from the end of the first fluid channel into the inlet of the closed flow cell.
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A system comprising:
- a plurality of reservoirs comprising a plurality of reagents, each reservoir of the plurality of reservoirs containing a reagent of the plurality of reagents;
- a pump configured to cause a flow of the plurality of reagents;
- an aspiration device coupled to a first end of a first fluid channel, the aspiration head configured to move about an X-axis, Y-axis and Z-axis, and to aspirate the plurality of reagents from the reagent reservoir into the first fluid channel,
- wherein the aspiration device is displaced outside the reagent reservoir between aspirating each reagent to draw at least one bubble into the first fluid channel such that the at least one bubble is disposed between adjacent reagents within the first fluid channel;
- a closed flow cell having an inlet and an outlet, wherein the inlet is coupled to a second end of the first fluid channel;
- and
- a de-bubbler coupled to the first fluid channel and disposed upstream of the closed flow cell, the de-bubbler configured to divert the at least one bubble from the first fluid channel.
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Dependencies are inferred from the claim text.