Nanoparticle with single site for template polynucleotide attachment

The method of claim 18, wherein the substrate comprises a plurality of nanowells and the oligonucleotides attached to the substrate are attached within the plurality of nanowells.

The method of any one of claims 18 through 20 further comprising synthesizing one or more substrate-attached copies selected from copies of the template polynucleotide, copies of the polynucleotides complementary to the template polynucleotide, and copies of both, wherein the substrate-attached copies extend from oligonucleotides attached to a substrate.

A nanoparticle, comprising

• a scaffold, a single template site for bonding a template polynucleotide to the scaffold selected from a covalent template bonding site and a noncovalent template bonding site, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites, wherein
• the scaffold comprises a dendrimer wherein the dendrimer comprises from 2 to 10 generations of constitutional repeating units, the constitutional repeating units comprise lysine wherein a lysine of an upstream generation forms a peptide bond with a first lysine of an immediately downstream generation and an isopeptide bond with a second lysine of the immediately downstream generation,
• the single template site extends from the C-terminal end of the lysine of the first generation of the dendrimer and the plurality of accessory sites extend from NH2 groups of lysines of the last generation of the dendrimer.

A method, comprising bonding a single template polynucleotide to the single template site of claim 19.