ORGAN REGENERATION METHOD UTILIZING iPS CELL AND BLASTOCYST COMPLEMENTATION

A non-human mammal having an abnormality associated with a lack of development of a target organ in a development stage, the mammal being produced by a method including the steps of:

• a) preparing an iPS cell derived from a different individual mammal that is an individual different from the non-human mammal;
• b) transplanting the iPS cell into a blastocyst stage fertilized egg of the non-human mammal;
• and
• c) developing the fertilized egg in a womb of a non-human surrogate parent mammal to obtain a litter.

A set for producing a target organ, the set comprising:

• A) a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage;
• and
• B) any one of
• an iPS cell derived from a different individual mammal that is an individual different from the non-human mammal, and
• a reprogramming factor and, if necessary, a somatic cell.

A method for producing any one of a target organ and a target body part, the method comprising the steps of:

• A) providing an animal which includes a deficiency responsible gene coding for a factor which causes a deficiency of any one of an organ and a body part and gives any one of no possibility of survival and difficulty in survival if the factor functions, and in which the any one of an organ and a body part is complemented by blastocyst complementation, the deficiency responsible gene coding for a factor which causes a deficiency of the any one of a target organ and a target body part;
• B) growing an ovum obtained from the animal into a blastocyst;
• C) introducing a target iPS cell into the blastocyst so as to produce a chimeric blastocyst, the target iPS cell having a desired genome capable of complementing a deficiency caused by the deficiency responsible gene;
• and
• D) producing an individual from the chimeric blastocyst, and then obtaining the any one of a target organ and a target body part from the individual.

A set for producing any one of a target organ and a target body part, the set comprising:

• A) a non-human animal which includes a gene coding for a factor which causes a deficiency of any one of an organ and a body part and gives any one of no possibility of survival and difficulty in survival if the factor functions, and in which the any one of an organ and a body part is complemented by complement;
• and
• B) any one of
• an iPS cell derived from a different individual mammal that is an individual different from the non-human mammal, and
• a combination of a reprogramming factor and, if necessary, a somatic cell.