Production and applications of ovarian and uterine organoids
AssigneeGameto, Inc
InventorsChristian KRAMME, Dina RADENKOVIC
Life of this patent
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Abstract
Featured are compositions, methods, and apparatuses for biosynthetic organs, or organoids, that replicate tissues of the human female reproductive system (e.g., ovarian or uterine tissues). In particular, the disclosure features methods of culturing or 2024/054644 producing organoids or uteroids from pluripotent stem or progenitor cells and using the resulting organoids or uteroids to screen for safe and effective pharmaceutical interventions for diseases of interest. Such methods and compositions are particularly useful for the treatment of patients undergoing hormone replacement therapy (HRT) and/or assisted reproduction technology (ART) procedures, such as in vitro fertilization and in vitro oocyte maturation. WO
Claims153
An organoid comprising a population of cells of a human female reproductive organ and an extracellular matrix (ECM) substrate, wherein the cells are obtained by differentiation, ex vivo, of one or more pluripotent stem or progenitor cells, and wherein the population of cells comprises:
- a) a plurality of ovarian granulosa cells, ovarian stroma cells, ovarian lutein cells, ovarian theca cells, and/or germ cells;
- and/or
- b) a plurality of uterine endometrial cells, uterine myometrial cells, and/or uterine perimetrial cells.
A method of producing an organoid comprising a population of human ovarian cells, the method comprising:
- a) introducing, into one or more pluripotent stem or progenitor cells, one or more nucleic acid molecules that collectively encode FOXL2, NR5A1, GATA4, RUNX1, and/or RUNX2, thereby differentiating the one or more pluripotent stem or progenitor cells into a population of ovarian granulosa cells, ovarian stroma cells, ovarian lutein cells, and/or ovarian theca cells;
- and
- b) contacting the cells resulting from (a) with an ECM substrate, optionally wherein the ECM substrate comprises one or more of collagen, an EGF, an elastin, a fibronectin, a vitronectin, a laminin, a cell adhesion protein, or a plant-derived protein or protein polymer.
A platform for engineering a human organoid replica for reproductive screening, the platform comprising:
- a) an engineered reproductive cell;
- b) a detector that measures a response by the engineered reproductive cell upon exposure of the engineered reproductive cell to a stimulus;
- c) a computing device in communication with the detector, wherein the computing further comprises:
- (i) at least a processor;
- and
- (ii) a memory communicatively connected to the processor, the memory containing instructions configuring the at least a processor to process stimulus data received from the detector and compare the stimulus data to a user profile.
A method for engineering a human organoid replica for reproductive screening, the method comprising:
- a) creating a user profile as a function of a reproductive cell relating to a user;
- b) receiving a plurality of human iPSCs;
- c) recapitulating, in vitro, the reproductive cell utilizing the plurality of human iPSCs;
- and
- d) generating, as a function of the recapitulated reproductive cell, a human organoid replica of an ovary.
Dependencies are inferred from the claim text.