# US 2026/0085367 A1 · Lamp assay system for detection of respiratory virus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2026/0085367 A1 - Document ID: US20260085367A1 - Status: Published application - Kind: A1 - Application number: 18/898,476 - Filed: 2024-09-26 - Published: 2026-03-26 - Earliest claimed: 2024-09-26 - Assignees: Delta Electronics Int’l (Singapore) Pte Ltd - Applicants: Delta Electronics Int’l (Singapore) Pte Ltd - Inventors: Zee Hong Goh; Weishi Zhang; Jia Hui Esther Boey - Web page: https://patentgrep.com/patent/US20260085367A1 ## CPC - C12Q 1/701: {Specific hybridization probes} - C12Q 1/6844: Nucleic acid amplification reactions - C12Q 2600/16: Primer sets for multiplex assays - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract A LAMP assay system for detection of a respiratory virus is disclosed. The LAMP system includes a LAMP reaction mixture having a primer kit, a strand-displacing polymerase and deoxyribonucleoside triphosphates for amplifying a target sequence. The primer kit includes at least one of a first primer set specific to SARS-CoV-2, a second primer set specific to Influenza A, and a third primer set specific to Influenza B. The first primer set includes primers having nucleotide sequences of SEQ ID NOs: 1 to 6 and SEQ ID NOs: 13 to 18. The second primer set includes primers having nucleotide sequences of SEQ ID NOs: 25 to 30. The third primer set includes primers having nucleotide sequences of SEQ ID NOs: 44 to 49. ## Claims ### Claim 1 A loop-mediated isothermal amplification (LAMP) assay system for detection of a respiratory virus, comprising a LAMP reaction mixture having a primer kit, a strand-displacing polymerase and deoxyribonucleoside triphosphates for amplifying a target sequence, wherein the primer kit comprises at least one of a first primer set specific to SARS-CoV-2, a second primer set specific to Influenza A, and a third primer set specific to Influenza B, wherein the first primer set comprises primers having nucleotide sequences of SEQ ID NOs: 1 to 6 and SEQ ID NOs: 13 to 18, wherein the second primer set comprises primers having nucleotide sequences of SEQ ID NOs: 25 to 30, and wherein the third primer set comprises primers having nucleotide sequences of SEQ ID NOs: 44 to 49. ### Claim 2 (depends on 1) The LAMP assay system according to claim 1, wherein the first primer set further comprises at least one of primers having nucleotide sequences of SEQ ID NOs: 7 to 12. ### Claim 3 (depends on 1) The LAMP assay system according to claim 1, wherein the first primer set further comprises at least one of primers having nucleotide sequences of SEQ ID NOs: 19 to 24. ### Claim 4 (depends on 1) The LAMP assay system according to claim 1, wherein the second primer set further comprises at least one of primers having nucleotide sequences of SEQ ID NOs: 31 to 37. ### Claim 5 (depends on 1) The LAMP assay system according to claim 1, wherein the second primer set further comprises at least one of primers having nucleotide sequences of SEQ ID NOs: 38 to 43. ### Claim 6 (depends on 1) The LAMP assay system according to claim 1, wherein the third primer set further comprises at least one of primers having nucleotide sequences of SEQ ID NOs: 50 to 59. ### Claim 7 (depends on 1) The LAMP assay system according to claim 1, wherein the primer kit further comprises an internal control primer set specific to β-actin, and the internal control primer set comprises primers having nucleotide sequences of SEQ ID NOs: 60 to 65. ### Claim 8 (depends on 1) The LAMP assay system according to claim 1, wherein the LAMP reaction mixture further comprises a pH indicating dye in a concentration ranged 0.08 to 0.3 mM. ### Claim 9 (depends on 8) The LAMP assay system according to claim 8, wherein the pH indicating dye is phenol red or neutral red. ### Claim 10 (depends on 1) The LAMP assay system according to claim 1, wherein the LAMP reaction mixture further comprises a lyoprotectant sugar in a concentration ranged 1 to 10% (w/v), wherein the lyoprotectant sugar is selected from the group consisting of trehalose, raffinose, dextran, mannitol and mixtures thereof. ### Claim 11 (depends on 10) The LAMP assay system according to claim 10, wherein the LAMP reaction mixture comprises 2 to 7.5% (w/v) of trehalose and 2 to 7.5% (w/v) of raffinose. ### Claim 12 (depends on 10) The LAMP assay system according to claim 10, wherein the LAMP reaction mixture comprises 2 to 7.5% (w/v) of trehalose, 2 to 7.5% (w/v) of raffinose, 1 to 2.5% (w/v) of dextran, and 1 to 5% (w/v) of mannitol. ### Claim 13 (depends on 1) The LAMP assay system according to claim 1, wherein the LAMP reaction mixture further comprises a magnesium salt in a concentration ranged 4 to 12 mM. ### Claim 14 (depends on 1) The LAMP assay system according to claim 1, further comprising an extraction-free lysis buffer, wherein the extraction-free lysis buffer comprises: potassium chloride in a concentration ranged 10 to 50 mM; ammonium sulfate in a concentration ranged 10 to 50 mM; and a detergent in a concentration ranged 0.5 to 6% (w/v), wherein the detergent is selected from the group consisting of 2-ethylhexan-1-ol;2-methyloxirane;oxirane (CAS number: 64366-70-7), secondary alcohol ethoxylate (CAS number: 84133-50-6), 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol (CAS number: 9002-93-1), 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethan-1-ol (CAS number: 9002-93-1), and {2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy}ethyl dodecanoate (CAS number: 9005-64-5) and mixtures thereof. ### Claim 15 (depends on 14) The LAMP assay system according to claim 14, wherein the LAMP reaction mixture is all-in-one lyophilized mixture and is rehydrated with the extraction-free lysis buffer to be ready for nucleic acid amplification and detection. ### Claim 16 (depends on 14) The LAMP assay system according to claim 14, wherein the detergent in the extraction-free lysis buffer is 0.5 to 6% (w/v) of 2-ethylhexan-1-ol;2-methyloxirane;oxirane (CAS number: 64366-70-7). ### Claim 17 (depends on 14) The LAMP assay system according to claim 14, wherein the detergent in the extraction-free lysis buffer is 0.5 to 6% (w/v) of secondary alcohol ethoxylate (CAS number: 84133-50-6). ### Claim 18 (depends on 14) The LAMP assay system according to claim 14, wherein the detergent in the extraction-free lysis buffer is 0.5 to 6% (w/v) of 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol (CAS number: 9002-93-1). ### Claim 19 (depends on 14) The LAMP assay system according to claim 14, wherein the detergent in the extraction-free lysis buffer is 0.5 to 6% (w/v) of 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethan-1-ol (CAS number: 9002-93-1). ### Claim 20 (depends on 14) The LAMP assay system according to claim 14, wherein the detergent in the extraction-free lysis buffer is 0.5 to 6% (w/v) of {2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy}ethyl dodecanoate (CAS number: 9005-64-5).