# US 2026/0085366 A1 · Psilocybe assay

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2026/0085366 A1
- Document ID: US20260085366A1
- Status: Published application
- Kind: A1
- Application number: 19/111,063
- Filed: 2023-09-11
- Published: 2026-03-26
- Earliest claimed: 2022-09-13
- Assignees: Medicinal Genomics Corporation
- Applicants: Medicinal Genomics Corporation
- Inventors: Kevin MCKERNAN
- Web page: https://patentgrep.com/patent/US20260085366A1

## CPC

- C12Q 1/6895: for plants, fungi or algae
- C12Q 1/689: for bacteria
- C12Q 2600/16: Primer sets for multiplex assays
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method for assaying putative Psilocybe spores, Psilocybe tissue, or a cultured host organism that expresses Psilocybe genes comprises obtaining a sample including nucleic acids, contacting the sample with primers for amplifying target nucleic acid sequences, amplifying any of the target nucleic acid sequences when present among the nucleic acids to obtain amplicons, and detecting the amplicons upon amplification of the target nucleic acid sequence. In embodiments, the primers include primers for amplifying (i) a bacterial target nucleic acid sequence, (ii) a Psilocybe target nucleic acid sequence, and/or (iii) a fungal target nucleic acid sequence. For instance, the primers include primers for amplifying a Psilocybecubensis target nucleic acid sequence and a target nucleic acid sequence from at least one other mushroom species subject to misidentification as Psilocybecubensis. A kit may include those primers and probes for detecting the amplicons amplified from any of those target nucleic acid sequences.

## Claims

### Claim 1

A method for discriminating Psilocybe cubensis from at least one other mushroom species subject to misidentification as Psilocybe cubensis, comprising:
obtaining a sample including nucleic acids collected from putative Psilocybe cubensis spores or tissue;
contacting the sample with primers for amplifying target nucleic acid sequences;
amplifying any of the target nucleic acid sequences when present among the nucleic acids to obtain amplicons; and
detecting the amplicons upon amplification of the target nucleic acid sequences,
wherein the primers include primers for amplifying a Psilocybe cubensis target nucleic acid sequence and a target nucleic acid sequence from the other mushroom species.

### Claim 2 (depends on 1)

The method of claim 1, wherein:
the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence and/or any of a Psilocybe cubensis PsiK, PsiM, PsiH, or PsiD target nucleic acid sequence; and
the target nucleic acid sequence from the other mushroom species includes any of an amatoxin target nucleic acid sequence or an Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim 3 (depends on 1)

The method of claim 1, wherein:
the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence and/or any of a Psilocybe cubensis PsiK, PsiM, PsiH, or PsiD target nucleic acid sequence; and
the target nucleic acid sequence from the other mushroom species includes an amatoxin target nucleic acid sequence and an Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim 4 (depends on 1)

The method of claim 1, wherein the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim 5 (depends on 1)

The method of claim 1, wherein the target nucleic acid sequence from the other mushroom species includes an amatoxin target nucleic acid sequence from an amatoxin-producing Galerina species.

### Claim 6 (depends on 1)

The method of claim 1, wherein the target nucleic acid sequence from the other mushroom species includes an Internal Transcribed Spacer (ITS) target nucleic acid sequence from a Psilocybe species linked to wood lovers paralysis (WLP).

### Claim 7 (depends on 1)

The method of claim 1, wherein the target nucleic acid sequence from the other mushroom species includes an Internal Transcribed Spacer (ITS) target nucleic acid sequence from any of Psilocybe azurescens, Psilocybe cyanescens, Psilocybe allenii, Psilocybe subaeruginosa, Psilocybe stunzii, Psilocybe pelliculosa, or Psilocybe ovoideocystidiata.

### Claim 8 (depends on 1)

The method of claim 1, wherein the target nucleic acid sequence from the other mushroom species includes an Internal Transcribed Spacer (ITS) target nucleic acid sequence from any of Psilocybe azurescens, Psilocybe cyanescens, Psilocybe allenii, or Psilocybe subaeruginosa.

### Claim 9 (depends on 1)

The method of claim 1, wherein detection of the amplicons includes quantification of the amplicons.

### Claim 10 (depends on 1)

The method of claim 1, wherein the amplicons amplified from the target nucleic acid sequence from the other mushroom species are quantified during detection.

### Claim 11 (depends on 1)

The method of claim 1, wherein the amplicons amplified from the Psilocybe cubensis target nucleic acid sequence are quantified during detection.

### Claim 12 (depends on 1)

The method of claim 1, wherein a polymerase chain reaction (PCR) is performed to amplify the target nucleic acid sequences.

### Claim 13 (depends on 1)

The method of claim 1, wherein a real-time polymerase chain reaction (qPCR) is performed to amplify the target nucleic acid sequences.

### Claim 14 (depends on 1)

The method of claim 1, wherein multiplex amplification is performed to amplify the target nucleic acid sequences.

### Claim 15 (depends on 1)

The method of claim 1, wherein probes are hybridized to the amplicons to detect the amplicons.

### Claim 16 (depends on 15)

The method of claim 15, wherein the probes include a fluorescent label.

### Claim at position 17

17-24. (canceled)

### Claim 25

A kit for discriminating Psilocybe cubensis from at least one other mushroom species subject to misidentification as Psilocybe cubensis, comprising:
primers for amplifying a Psilocybe cubensis target nucleic acid sequence and a target nucleic acid sequence from the other mushroom species; and
probes for detecting amplicons of the Psilocybe cubensis target nucleic acid sequence and the target nucleic acid sequence from the other mushroom species,
wherein the probes include a fluorescent label.

### Claim 26

(canceled)

### Claim 27

(canceled)

### Claim 28 (depends on 25)

The kit of claim 25, wherein:
the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence and/or any of a Psilocybe cubensis PsiK, PsiM, PsiH, or PsiD target nucleic acid sequence; and
the target nucleic acid sequence from the other mushroom species includes any of an amatoxin target nucleic acid sequence or an Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim 29 (depends on 25)

The kit of claim 25, wherein:
the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence and/or any of a Psilocybe cubensis PsiK, PsiM, PsiH, or PsiD target nucleic acid sequence; and
the target nucleic acid sequence from the other mushroom species includes an amatoxin target nucleic acid sequence and an Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim 30 (depends on 25)

The kit of claim 25, wherein the Psilocybe cubensis target nucleic acid sequence includes a Psilocybe cubensis Internal Transcribed Spacer (ITS) target nucleic acid sequence.

### Claim at position 24

31-85. (canceled)