Using Multiple Heteroresistance to Guide Combination Antimicrobial Regimens
AssigneeEmory University
InventorsDavid Weiss, Victor Band, David Hufnagel
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Abstract
This disclosure contemplates detecting antimicrobial heteroresistance to guide antibiotic or other antimicrobial therapy. In certain embodiments, this disclosure contemplates avoiding a monotherapy with antibiotics to which an individual bacterial isolate is heteroresistant, because this can lead to treatment failure. Thus, in certain embodiments, one uses a combination of antibiotics to which a bacterium is individually heteroresistant.
Claims20
A method of determining whether a microbial culture is heteroresistant to antimicrobial agents and treating a microbial infection with a combination of heteroresistant antimicrobial agents comprising:
- obtaining a sample from a subject diagnosed with a microbial infection;
- growing microbes from said sample providing a microbial colony;
- contacting a plurality of individual antimicrobial agents with samples of the microbial colony providing individual contacted microbial cultures for each antimicrobial agent in the library;
- determining whether the individual contacted microbial cultures for each antimicrobial agent in the library is either,
- i) resistant to the antimicrobial agent,
- ii) heteroresistant to the antimicrobial agent, or
- iii) susceptible to the antimicrobial agent and
- administering to said subject in need of treatment an effective amount of a combination ofheteroresistant antimicrobial agents for treatment, wherein each antimicrobial agent in the combination of antimicrobial agents for treatment is determined from the individual contacted microbial cultures to be heteroresistant to each antimicrobial agent in said combination of heteroresistant antimicrobial agents for treatment.
Dependencies are inferred from the claim text.