New polymers and methods
AssigneeBiome Bioplastics Limited
InventorsPaul Law, Paul Mines, Timothy Bugg
Life of this patent
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Abstract
The present invention relates to processes for the formation of pyridinedicarboxylic acid (PDCA), in particular, 2,4-pyridinedicarboxylic acid (2,4-PDCA) and 2,5-pyridinedicarboxylic acid (2,5-PDCA), and mono- and diester derivatives thereof, from 3,4-dihydroxybenzoic acid, via a biocatalytic reaction using, for example, a protocatechuate dioxygenase such as protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase, and a nitrogen source. The invention also relates to copolymers that comprise the pyridinedicarboxylic acid monomers and derivatives thereof, processes for the formation of the copolymers and uses for the copolymers.
Claims25
-38. (canceled)
A process comprising combining:
- (a) a microorganism modified to express a protocatechuate dioxygenase, and
- (b) a carbon source;
- to produce 2,5-pyridinecarboxylic acid or 2,4-pyridinecarboxylic acid.
A method comprising:
- combining in a bioreactor
- (a) a nitrogen source;
- (b) a carbon source;
- and
- (c) a microorganism modified to express a protocatechuate dioxygenase;
- fermenting the microorganism, nitrogen source and carbon source under suitable conditions and for a suitable time to produce acid 2,5-pyridinecarboxylic acid or 2,4-pyridinecarboxylic acid;
- and
- separating the 2,5-pyridinecarboxylic acid or 2,4-pyridinecarboxylic acid from the fermentation medium.
A process comprising combining:
- (a) a microorganism modified to express protocatechuate 2,3-dioxygenase or protocatechuate 4,5-dioxygenase, wherein the microorganism comprises a praA gene that encodes the protocatechuate 2,3-dioxygenase, or the ligAB genes that encode protocatechuate 4,5-dioxygenase;
- and
- (b) a carbon source;
- to produce 2,5-pyridinecarboxylic acid or 2,4-pyridinecarboxylic acid.
Dependencies are inferred from the claim text.