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AssigneeEnzymaster (Ningbo) Bio-Engineering Co., Ltd
InventorsHaibin CHEN, Yafang LIANG, Yingxin ZHANG, Zekun XIA, Ameng ZHOU, Marco BOCOLA, Junpeng XU, Xiao LUO, Jingxian MAO, Ruimei HONG, Xinwei WU, Lei SUN, Yixin DAI, Songxue WANG, Qinli PENG
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The present disclosure provides an engineered ketoreductase polypeptide capable of preparing chiral alcohols, especially for catalyzing the reduction of ethyl 4-chloroacetoacetate to produce ethyl (R)-4-chloro-3-hydroxybutyrate. The polypeptides has high catalytic activity, high stereoselectivity, better thermal stability and solvent tolerance; the reaction process using engineered ketoreductase polypeptides may have a substrate loading of up to 426 g/L and a conversion of up to 99%.
An engineered ketoreductase polypeptide that catalyzes the reduction of ethyl 4-chloroacetoacetate to produce ethyl (R)-4-chloro-3-hydroxybutyrate with an ee value of at least 99%, the polypeptide comprising an amino acid sequence having at least 90% sequence identity to the reference sequence SEQ ID NO:
An engineered ketoreductase polypeptide, which is capable of converting ethyl 4-chloroacetoacetate to ethyl (R)-4-chloro-3-hydroxybutyrate with better catalytic activity and/or stability than SEQ ID NO:
Dependencies are inferred from the claim text.