Feeder cells, cell sheet, production method for feeder cells and cell sheet, and method of maintaining or proliferating cells using feeder cell
AssigneesDai Nippon Printing Co., Ltd., National Center for Child Health and Development
InventorsKenichi MORINAGA, Akihiro UMEZAWA
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Abstract
A feeder cell for maintaining or proliferating, by a two-dimensional culture, a cell deemed to be difficult to maintain and proliferate by a two-dimensional culture; and a method of producing the feeder cell. A fibroblast-like cell is produced from a pluripotent stem cell, and derived from an intestinal structure containing an endoderm-derived cell and a mesoderm-derived cell is proliferated, isolated, and used as a feeder cell.
Claims28
A method of producing a feeder cell for maintaining or proliferating a cell, comprising:
- (A) a step of preparing an intestinal structure produced from a pluripotent stem cell, and containing an endoderm-derived cell and a mesoderm-derived cell;
- (B) a step of culturing the intestinal structure to proliferate a fibroblast-like cell derived from the intestinal structure;
- and
- (C) a step of isolating the fibroblast-like cell to obtain a feeder cell.
A fibroblast-like cell which is positive for PDGFRA and CD81, and expresses Foxl1 and GREM1.
A cell which expresses at least one gene selected from the group consisting of DCN, ACTA2, and NOG, and expresses the genes, ADAMTS19, ANO1, BLID, LOC100507053, LHX8, SFRP4, AHRR, SLC14A1, FMO3, PZP, ABCG4, EYA2, TMEM92-AS1, ANO10, EGFL6, SNCAIP, LGSN, PCDHB15, and KRTAP1-5.
Dependencies are inferred from the claim text.