Cell culture method and production method for product
AssigneeFUJIFILM Corporation
InventorsAtsushi INADA, Shinichi NAKAI, Katsuhiro SHIMONO
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Abstract
An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed. According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells/mL or more and 400×106 cells/mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.
Claims11
A cell culture method of culturing cells at a cell density of 30×106 cells/mL or more and 400×106 cells/mL or less in a culture solution, the method comprising:
- step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution, or
- step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower,
- wherein step A is performed in a case where the poloxamer concentration in the foam liquid constituting the foam is 6 times or more the poloxamer concentration in the culture solution, or
- step B is performed in a case where the viscosity of the foam liquid constituting the foam is 1.5 mPa·s or more under the conditions of 35° C. or higher and 38° C. or lower.
A production method for a product, comprising:
- culturing cells at a cell density of 30×106 cells/mL or more and 400×106 cells/mL or less in a culture solution,
- wherein the method includes
- step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution, or
- step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower,
- wherein step A is performed in a case where the poloxamer concentration in the foam liquid constituting the foam is 6 times or more the poloxamer concentration in the culture solution, or
- step B is performed in a case where the viscosity of the foam liquid constituting the foam is 1.5 mPa·s or more under the conditions of 35° C. or higher and 38° C. or lower.
Dependencies are inferred from the claim text.