Adaptation and process optimization of microorganisms for growth in hemicellulosic derived carbohydrates

A method of making microorganisms with increased xylose consumption comprising

• a. providing xylose-consuming microorganisms comprising two or more copies of a nucleic acid sequence encoding xylose isomerase and two or more copies of a nucleic acid sequence encoding a xylose kinase;
• b. culturing the microorganisms in a first culture medium comprising xylose for at least 3 days;
• c. harvesting a portion of the microorganisms from the first culture medium after culture step (b);
• d. culturing the harvested portion of microorganisms in a second culture medium comprising xylose for at least 3 days;
• e. harvesting a portion of the microorganisms from the second culture medium after culture step (d);
• f. repeating culturing and harvesting steps (d) and (e) at least two times in a third culture medium and a fourth culture medium;
• g. isolating the harvested microorganisms from step (f), wherein the isolated microorganisms have increased xylose consumption rates compared to control xylose-consuming microorganisms.