Method for operating a bioprocess installation for production of a bioproduct

A method for operating a bioprocess installation for production of a bioproduct, wherein the bioprocess installation comprises a source receptacle for cell cultivation, a harvest receptacle for bioproduction and a clarification setup with a centrifuge,

• wherein the source receptacle is operated in a cyclical production mode,
• wherein the cyclical production mode comprises, in this order, the steps of:
• a) starting the cyclical production mode in the source receptacle with initial cells and cultivation medium,
• b) cultivating the cells in the source receptacle, thereby obtaining a cell broth comprising cultivated cells, whereby the source receptacle and the cultivation medium are configured to provide source culture environment conditions for the cultivation of the cells,
• c) discharging a discharge fraction of the cell broth from the source receptacle,
• d) combining a restart fraction of the cell broth with fresh cultivation medium and repeating step b),
• e) repeating steps c) and d) at least once and/or
• f) discharging the cell broth obtained from step d) from the source receptacle stopping the cyclical production mode, obtaining a discharge fraction,
• wherein the method further comprises the steps of:
• i) transferring the discharge fraction to the clarification setup and centrifuging the discharge fraction via the centrifuge thereby separating the discharge fraction into at least a centrifuged discharge fraction and supernatant,
• ii) transferring at least part of the centrifuged discharge fraction from step i) and fresh cultivation medium into the harvest receptacle and operating the harvest receptacle in a production mode, whereby the harvest receptacle and the cultivation medium are configured to provide harvest culture environment conditions, producing the bioproduct in the harvest receptacle,
• wherein steps i) and ii) are executed at least twice with discharge fractions from an execution of step c) and/or step f).