Methods of detecting immune cells
AssigneeBiosceptre (Aust) Pty Ltd
InventorPatrick Schlegel
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Abstract
The invention relates to fusion proteins and uses thereof for obtaining and isolating enriched populations of immune cells. Exemplified fusion proteins include those comprising a dysfunctional purinergic P2X7 receptor (P2X7R) epitope moiety and a Fc region of an antibody.
Claims33
A fusion protein comprising:
- (i) a dysfunctional P2X7 receptor epitope moiety;
- and
- (ii) an Fc region of an antibody, wherein the Fc region has a reduced affinity for an Fc receptor compared to wild-type or naturally occurring Fc regions.
An in vitro method for detecting an immune cell expressing a receptor that comprises an antigen binding domain for binding to dysfunctional P2X7 receptor, the method comprising:
- (i) providing a biological sample from a patient who has received a treatment with immune cells, preferably immune effector cells, wherein the cells express a receptor comprising an antigen binding domain for binding to dysfunctional P2X7 receptor;
- (ii) contacting the sample with a polypeptide, wherein the polypeptide comprises an epitope of dysfunctional P2X7 receptor which is recognised by the antigen binding domain of the receptor, and wherein the polypeptide comprises a detection moiety for enabling detection of the polypeptide;
- to thereby allow formation of a complex of the polypeptide bound to the cells;
- (iii) detecting the complex,
- thereby detecting an immune cell expressing a receptor having an antigen binding domain for binding to dysfunctional P2X7 receptor.
An in vitro method for detecting immune cells expressing a chimeric antigen receptor (CAR) for binding to dysfunctional P2X7 receptor, the method comprising:
- (i) providing a biological sample from a patient who has received a treatment with immune cells, preferably immune effector cells, comprising a chimeric antigen receptor (CAR) for binding to dysfunctional P2X7 receptor;
- (ii) contacting the sample with a polypeptide, wherein the polypeptide comprises an epitope of dysfunctional P2X7 receptor which is recognised by the CAR, and wherein the polypeptide comprises a detection moiety for enabling detection of the polypeptide;
- to thereby allow formation of a complex of the polypeptide bound to the cells;
- (iii) detecting the complex,
- thereby detecting immune cells expressing a chimeric antigen receptor (CAR) for binding to dysfunctional P2X7 receptor.
Dependencies are inferred from the claim text.