Compositions and methods for producing pea plants with enhanced yield, height, and/or lodging phenotype
AssigneeConfluence Genetics, LLC
InventorsHerbert Wolfgang Goettel, Janice R. Kofsky
Life of this patent
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Abstract
Provided herein are methods and compositions for producing pea plants having a desirable yield, height, and/or lodging phenotype using marker-assisted selection. The disclosure further provides methods and compositions for introgressing one or more loci comprising at least an allele linked to the yield, height, and/or lodging QTL, thus producing pea plants with a desirable yield, height, and/or lodging phenotype.
Claims29
A method of producing a population of pea plants or seeds with a desirable yield, height, and/or lodging phenotype, said method comprising:
- (a) genotyping a first population of pea plants or seeds for the presence of at least one yield, height, and/or lodging molecular marker that is within 20 centimorgans of one or more yield, height, and/or lodging Quantitative Trait Locus (QTLs) selected from the group consisting of yield chr2-2 (Ps02_417306163), yield chr5-1 (Ps05_117545329), yield chr5-2 (Ps05_604790207), lodging chr2-1 (Ps02_415727951), lodging chr5-1 (Ps05_608256598), lodging chr6-1 (Ps06_5054240), height chr2-2 (Ps2_390480167), height chr3-1 (Ps03_74158043), height chr3-2 (Ps03_496270063), height chr4-1 (Ps04_192115819), height chr6-1 (Ps06_38889673), height chr7-1 (Ps07_115631962), height chr7-2 (Ps07_129458029), height chr7-3 (Ps07_256289873), Ps01_12111263, Ps01_21627814, Ps01_80920094, Ps01_165828422, Ps01_172888276, Ps01_274311344, Ps02_277385615, Ps01_306049485, Ps01_345383217, Ps01_414516587, Ps02_389051201, Ps02_406013484, Ps02_415945622, Ps03_98286373, Ps04_33913844, Ps04_383816669, Ps04_445153308, Ps05_48702172, Ps05_237258346, Ps05_318938718, Ps05_320108884, Ps05_526760209, Ps05_536892706, Ps06_32259152, Ps06_43753843, Ps06_105337899, Ps06_127479778, Ps06_367630606, Ps06_427519500, Ps07_9801763, Ps07_39866790, Ps07_129103458, Ps07_142955054, Ps07_173064631, Ps07_173321635, Ps07_278403545, Ps07_303005109, Ps07_481516842, Ps01_279286967, Ps01_405953972, Ps02_66314510, Ps02_146578429, Ps03_114813976, Ps03_120645295, Ps03_230771576, Ps03_253149185, Ps03_512230003, Ps03_531239107, Ps03_71303964, Ps04_69656387, Ps04_144177304, Ps04_156326296, Ps04_260958274, Ps04_264937409, Ps04_374148702, Ps04_453044385, Ps05_3468595, Ps05_223782536, Ps05_352490739, Ps05_473638215, Ps05_481936646, Ps05_528041290, Ps05_551582581, Ps05_583707944, Ps05_612824899, Ps05_616716582, Ps06_12615016, Ps06_33377536, Ps06_349137016, Ps06_425993896, Ps07_133823647, Ps07_194142598, Ps07_195412515, Ps01_68070331, Ps02_233036770, Ps02_342684459, Ps02_426672885, Ps02_433458109, Ps03_94359486, Ps03_104956337, Ps03_118892995, Ps03_195398925, Ps03_205809630, Ps03_241957626, Ps03_242777502, Ps03_251323511, Ps03_374110266, Ps03_437895589, Ps03_493092288, Ps04_205866569, Ps04_389245228, Ps04_430139407, Ps04_448803743, Ps05_5262178, Ps05_160013078, Ps05_174010831, Ps05_277684648, Ps06_213382570, Ps06_236678213, Ps06_398588276, Ps06_472058597, Ps06_478057404, Ps07_58281807, Ps07_105730908, Ps07_112377551, Ps07_114916793, Ps07_213648906, Ps07_234681662, Ps07_238767894, and Ps07_489306364;
- (b) selecting from the first population one or more pea plants or seeds comprising one or more yield, height, and/or lodging alleles having the at least one yield, height, and/or lodging molecular marker;
- and
- (c) producing a second population of progeny pea plants or seeds from the selected one or more pea plants or plants grown from the selected seeds, wherein the second population of progeny pea plants or seeds comprises the one or more yield, height, and/or lodging alleles having the at least one yield, height, and/or lodging molecular marker,
- and wherein the second population of progeny pea plants or seeds are pea plants or seeds with a desirable yield, height, and/or lodging phenotype, thereby producing a population of pea plants or seeds with a desirable yield, height, and/or lodging phenotype.
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A method of introgressing a yield, height, and/or lodging QTL, the method comprising:
- (a) crossing a first pea plant comprising a yield, height, and/or lodging QTL with a second pea plant of a different genotype to produce one or more progeny plants or seeds;
- and
- (b) selecting a progeny plant or seed comprising one or more yield, height, and/or lodging alleles of a polymorphic locus linked to the yield, height, and/or lodging QTL,
- wherein the polymorphic locus corresponds to a chromosomal segment comprising any marker within a genomic region between positions:
- 386683682-424059129 of chromosome 2;
- 57407964-130197960 of chromosome 5;
- 541653217-end of chromosome 5;
- 387250594-427410060 of chromosome 2;
- 547677746-end of chromosome 5;
- start-17888751 of chromosome 6;
- 354324649-402078389 of chromosome 2;
- 18873917-69320372 of chromosome 3;
- 273327332-422042733 of chromosome 3;
- 118944413-200876233 of chromosome 4;
- 17641704-56905305 of chromosome 6;
- 79787890-153689044 of chromosome 7;
- 91738904-165762735 of chromosome 7;
- 207998578-360550624 of chromosome 7;
- scaffold00011;
- 73778015-347862320 of chromosome 1 LG6;
- 287196406-405820442 of chromosome 2 LG1;
- scaffold05541;
- 22316149-374998060 of chromosome 4 LG4;
- 35018291-496475925 of chromosome 5 LG3;
- 4102 if scaffold00839;
- 332130985-426328693 of chromosome 6 LG2;
- 8316115-481251260 of chromosome 7 LG7;
- scaffold02229;
- 122338217-340372022 of chromosome 1 LG6;
- 12117951-41766109 of chromosome 2 LG1;
- 55052537-420398638 of chromosome 3 LG5;
- scaffold06018;
- scaffold03592;
- super-scaffold8606;
- 47145729-440853776 of chromosome 4 LG4;
- scaffold02661;
- 3579419-570360377 of chromosome 5LG3;
- super-scaffold9678;
- 2560210-348314640 of chromosome 6 LG2;
- 191955823-63397608 of chromosome 7 LG7;
- 63453702 of chromosome 1 LG6;
- 96628783-415226641 of chromosome 2 LG1;
- scaffold05147;
- 403366757 of chromosome 4 LG4;
- scaffold00254;
- 811842-294741093 of chromosome 3 LG5;
- scaffold03249;
- 173468835-440508849 of chromosome 4 LG4;
- 362378-229071963 of chromosome 5 LG3;
- scaffold01526;
- 157000374-469077464 of chromosome 6 LG2;
- scaffold01649;
- or 750954-487220871 of chromosome 7 LG7 of a Pisum sativum genome.
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A nucleic acid molecule for detecting a yield, height, and/or lodging molecular marker in a Pisum sativum genome, wherein the nucleic acid molecule comprises at least 15 nucleotides that include or are immediately adjacent to the marker, wherein the nucleic acid molecule is at least 90 percent identical to a sequence of the same number of consecutive nucleotides in either strand of DNA that include or are immediately adjacent to the marker.
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Dependencies are inferred from the claim text.