Fabrication of fluorescent nanoparticles and their conjugates for in vitro and in vivo diagnostics
OwnerNi XIE, Benzhong TANGinventors
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Abstract
Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen™ Bright Green.
Claims30
A nanoparticle with solid state luminescence quantum yield higher than 20%, comprising luminophores entangled in a mesh of polymer.
A method for fabricating nanoparticles with solid state luminescence quantum yield higher than 20%, comprising the steps of:
- a. dissolving luminophores in one or more monomers to form a monomer mixture with 1-500 g/L of luminophores in said monomer mixture;
- b. dissolving an initiator in deionized water to form an initiator solution;
- c. dissolving a surfactant in deionized water to form a reaction mixture;
- d. adding said monomer mixture and said initiator solution to said reaction mixture;
- and
- e. heating the total mixture of step (d) until it turns milky white, wherein said nanoparticles are formed.
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Dependencies are inferred from the claim text.