Three dimensional immunomechanical organoid model
AssigneeUniversity of Notre Dame du Lac
InventorsAlice BURCHETT, Meenal DATTA
Life of this patent
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Abstract
A 3D immunomechanical model of myeloid cells for use in basic research and drug screening that allows for observation of the myeloid-mechanical interaction in the context of a range of different mechanical and immunological-related diseases. The model is also suitable for applications such as high-throughput drug screening and experiments in a microgravity environment such as low earth orbit.
Claims17
A method of testing a stimulus on an organoid comprising:
- subjecting an organoid embedded in a hydrogel to a stimulus, wherein the organoid comprises a first population of live myeloid cells;
- and
- measuring an immunological and/or physical state of the organoid to the stimulus as compared to an immunological and/or physical state of the organoid prior to contact with the stimulus or in the absence of the stimulus, wherein the stimulus comprises one or more of:
- a) a compressive force;
- b) one or more compounds of interest;
- and
- c) microgravity.
An organoid comprising a first population of live myeloid cells, and optionally, at least a second population of live cells that are not myeloid cells, wherein the organoid is embedded within a hydrogel comprising agarose, and optionally, gelatin.
Dependencies are inferred from the claim text.