Use of vapor deposition coated flow paths for improved analytical analysis
AssigneeWaters Technologies Corporation
InventorsXiaoxiao Liu, Anna Boardman, Mathew DeLano, Matthew A. Lauber
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Abstract
A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I. R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20—, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.
Claims20
A bioinert sample preparation device comprising wetted surfaces defining a fluidic flow path extending within an interior of the sample preparation device,
- wherein a material forming the wetted surfaces of the fluidic flow path prior to coating is formed of a polymeric material;
- and
- wherein at least a portion of the wetted surfaces of the fluidic flow path is coated with a coating comprising a layer formed by infiltrating vaporized 3-glycidyloxypropyl)trimethoxysilane, n-decyltrichlorosilane, trimethylchlorosilane, trimethyldimethylaminosilane, or methoxy-polyethyleneoxy(3)silane.
A method of tailoring a polymeric fluidic flow path for sample preparation of a liquid sample comprising a biomolecule, the method comprising:
- (a) pretreating wetted surfaces of the polymeric fluidic flow path with a plasma;
- (b) infiltrating at least one vaporized agent selected from the group consisting of:
- bis(trichlorosilyl)ethane, bis(trimethoxysilyl)ethane, 3-glycidyloxypropyl)trimethoxysilane, n-decyltrichlorosilane, trimethylchlorosilane, trimethyldimethylaminosilane, and methoxy-polyethyleneoxy(3)silane into the polymeric fluidic flow path;
- and
- (c) controlling temperature and pressure to deposit a coating on the wetted surfaces, the coating having a thickness of at least 100 Å and a contact angel of at least 15°.
Dependencies are inferred from the claim text.