Methods for nucleic acid analysis
AssigneeUltima Genomics, Inc.
InventorsGilad ALMOGY, Florian OBERSTRASS, Omer BARAD, Chandan SHEE
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Abstract
The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead.
Claims11
1-93. (canceled)
A method for linked read sequencing, comprising:
- (a) attaching a first adapter and a second adapter to a template nucleic acid molecule comprising a first strand and a second strand to generate a complex, wherein the first adapter comprises a stem loop configuration and the second adapter comprises a Y-shaped configuration, wherein the first adapter comprises a sequencing primer sequence or a complement thereof, wherein the first strand is linked to the second strand via the first adapter in the complex;
- and
- (b) immobilizing the complex, or a derivative thereof, to a substrate surface;
- and
- (c) hybridizing a sequencing primer to the sequencing primer sequence or the complement thereof, of the complex or the derivative thereof immobilized to the substrate surface, and extending the sequencing primer to sequence the first strand or the second strand.
Dependencies are inferred from the claim text.