Probiotics designed to express and secrete akkermansia muciniphila tars, and a vector for producing the same
AssigneeKorea Research Institute of Bioscience and Biotechnolgy
InventorsMyung Hee KIM, Shinhye Park, Su Man Kim, Seungho Hwang
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Abstract
The present invention relates to: a transformed strain expressing and secreting Akkermansia muciniphila TARS (AmTARS); and a vector for producing same. More specifically, the present invention relates to an Escherichia coli Nissle 1917 (EcN) strain expressing AmTARS, a composition comprising same, a novel vector for producing same, and an EcN transformation method using same.
Claims20
An Escherichia coli Nissle 1917 (EcN) strain transformed with a vector comprising a nucleic acid encoding TARS of Akkermansia muciniphila (AmTARS).
A vector comprising a 5′-homology arm, a promoter, a ribosome binding site consisting of the nucleotide sequence of SEQ ID NO:
- 2, a nucleic acid encoding TARS of Akkermansia muciniphila (AmTARS), and a 3′-homology arm,
- wherein the 5′-homology arm comprises a nucleotide sequence corresponding to a portion of the exo gene and exo/cea intergenic region of Escherichia coli Nissle 1917 (EcN),
- and the 3′-homology arm comprises a nucleotide sequence corresponding to a portion of the exo/cea intergenic region and cea gene of EcN.
Dependencies are inferred from the claim text.