HLA Triple Knockout Human Follicle Cells for Enhanced Hair Implantation Therapy with Integrated Biosensor System
OwnerReem Mahratinventor
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Abstract
The present invention discloses a novel method for developing Human HLA-a/b/c triple knockout hair follicle stem cells (HFSCs) intended for promoting HFSCS transplantation. The method involves precise genetic modification techniques to knockout HLA-a/b/c genes, facilitating enhanced compatibility and effectiveness in hair follicles transplantation. The inventive approach aims to address challenges (donor dependent and large scar) in hair implantation therapy by leveraging advanced gene-editing and stem cell technologies.
Claims11
A method for producing immuno-tolerant hair follicle stem cells (HFSCs), comprising:
- a. isolating and culturing HFSCs;
- b. genetically modifying the HFSCs to create HLA-a/b/c triple knockout HFSCs;
- c. a biosensor system established to select undifferentiated HFSCs, wherein the biosensor system comprises a CD105 linked GFP and a CD45 linked RFP.
A system for producing immuno-tolerant hair follicle stem cells (HFSCs), comprising:
- a. a culture medium for HFSCs;
- b. a CRISPR/Cas9 gene-editing toolkit for creating HLA-a/b/c triple knockout HFSCs;
- c. a biosensor system for selecting undifferentiated HFSCs.
Dependencies are inferred from the claim text.