Brassica cytoplasmic male sterility (cms) fertility restorer nucleic acids, markers, methods, and zygosity assays
AssigneePioneer Hi-Bred International, Inc.
InventorsSARAH ATWOOD, KEVIN A. FENGLER, SIVA S. AMMIRAJU JETTY, JONATHAN MYRVOLD
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Abstract
This disclosure concerns methods and compositions for identifying plants that have an Ogura CMS restorer of fertility phenotype. Some aspects concern a zygosity assay to identify, select, and/or construct Ogura CMS restorer of fertility plants and germplasm. Particular aspects concern a zygosity assay to maintain an Ogura fertility restoration system for hybrid canola production. This disclosure also concerns plants comprising an Ogura fertility restoration system for hybrid canola production generated by methods utilizing the zygosity assay described herein.
Claims20
A method of identifying a B. napus plant, cell, or germplasm thereof comprising a truncated Ogura Rf segment that includes the Rfo gene, the method comprising:
- a. obtaining a nucleic acid sample from a B. napus plant, cell, or germplasm;
- and
- b. screening the sample for the truncated Ogura Rf segment on chromosome N19, wherein the segment is less than about 5,000 kb in length, and wherein the presence of the segment contributes to a fertility restorer phenotype in B. napus.
A PCR assay method for determining zygosity of a truncated Ogura Rf segment in a B. napus plant, cell or germplasm, the method comprising:
- a. performing a first PCR assay using a first probe, a first forward primer, and a first reverse primer on a polynucleotide from a B. napus plant sample, wherein the first probe is SEQ ID NO:3;
- b. performing a second PCR assay using a second probe, a second forward primer, and a second reverse primer on the polynucleotide sample, wherein the second probe is SEQ ID NO:6;
- c. quantifying the first probe and the second probe;
- and,
- d. comparing the quantified first probe and the quantified second probe of the first PCR assay and the second PCR assay to determine the zygosity.
A homozygous restorer B. napus plant, wherein the plant comprises the truncated Ogura Rf segment that is about 4,900 kb and does not comprise the wildtype N19 genomic segment that is about 2,100 kb.
Dependencies are inferred from the claim text.