Ras biosensors
AssigneeThe Regents of the University of California
InventorsJin Zhang, Ryan Weeks
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Abstract
Provided herein are, inter alia, biosensor recombinant proteins (e.g. ratiometric Ras activity reporter (RasAR)), which provide quantitative measurement of Ras activity in living cells with high spatiotemporal resolution. The biosensor recombinant proteins provided herein include, for example, a novel pseudoligand and are, inter alia, useful for detecting a Ras protein and activity of a Ras protein in a cell.
Claims70
A recombinant protein comprising a first fluorophore, a ligand binding domain, a pseudoligand domain, and a second fluorophore.
A method for detecting a Ras protein in a cell, the method comprising:
- (a) contacting a cell with an expression vector comprising an isolated nucleic acid, wherein the isolated nucleic acid encodes a recombinant protein comprising:
- a first fluorophore, a ligand binding domain, a pseudoligand domain, and a second fluorophore;
- (b) transducing the cell with the expression vector;
- (c) allowing the cell to express the recombinant protein and bind to the Ras protein within the cell;
- and
- (d) detecting a fluorescent signal form the recombinant protein thereby detecting the Ras protein in the cell.
Dependencies are inferred from the claim text.