US 2026/0071968 A1Published application

New method for detecting four sulfur-containing pesticides based on gold and silver nano-mimetic enzyme materials and application thereof

AssigneeSouth-Central Minzu University

InventorsHaiyan FU, Xueqing Zeng, Hengye Chen, Yuanbin She, Wanjun Long, Wei Lan

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Abstract

A new colorimetric detection method for sulfur-containing pesticides with nano-mimetic enzyme is provided. The synthesized gold-silver bimetallic nanocluster (Au—Ag NCs) has quasi-peroxidase activity and catalyze hydrogen peroxide to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue products under acidic conditions. Based on the electrostatic interaction between Au—Ag NCs and four sulfur-containing pesticides, the exposed Au and Ag elements further combine with S in pesticide molecules to form bonds, and Au—Ag NCs loses the quasi-peroxidase activity and blocks the catalytic oxidation process.

Claims8

Dependent claims

A preparation method of nano-quasi-peroxidase, wherein the nano-quasi-peroxidase is a gold-silver bimetallic nanocluster Au—Ag NCs, and the Au—Ag NCs has a bowl-shaped hollow structure with a rough surface and a negatively charged outer layer;

wherein the preparation method comprises following steps:
(1) heating and boiling a silver nitrate solution in dark, quickly adding a trisodium citrate aqueous solution while stirring, uniformly mixing, stirring and boiling in the dark for 1 h;
and
(2) after slowly adding a chloroauric acid aqueous solution at 90° C. with stirring, continuing to stir at 90° C. in the dark for 45 min;
and after completing a reaction, cooling a reaction system to room temperature, filtering an obtained solution, and then purifying to prepare the Au—Ag NCs.

2 more elements

Dependencies are inferred from the claim text.