Determination apparatus and control method for determination apparatus
AssigneeCanon Kabushiki Kaisha
InventorsAKIRA TERAO, YUKI YONETANI, MAKOTO KAWAGUCHI
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Abstract
A determination apparatus comprises an acquisition unit configured to acquire a Raman spectral image captured by an image capturing apparatus that captures stimulated Raman scattering light from a biological cell irradiated with excitation light, a generation unit configured to generate a spectral subtraction image of a pair of images corresponding to different wavenumber shift bands of the Raman spectral image, and a determination unit configured to determine a state of a cell based on the spectral subtraction image.
Claims23
A determination apparatus comprising:
- an acquisition unit configured to acquire a Raman spectral image captured by an image capturing apparatus configured to capture stimulated Raman scattering light from a biological cell irradiated with coherent primary light;
- a generation unit configured to generate spectral difference information for emphasizing a Raman scattering light signal corresponding to one of different wavenumber shift bands of the Raman spectral image with respect to a Raman scattering light signal corresponding to the other of the different wavenumber shift bands;
- a cell region identification unit configured to acquire cell identification information that identifies a cell region in a field of view of the Raman spectral image;
- and
- a determination unit configured to determine a state of the cell based on the spectral difference information and the cell identification information.
A method for controlling a determination apparatus that comprises an acquisition unit configured to acquire a Raman spectral image, a generation unit configured to generate spectral difference information that emphasizes a Raman signal of a first component with respect to a Raman signal of a second component, a cell region identification unit configured to identify a cell region, and a determination unit configured to determine a state of a cell contained in a sample, the method comprising:
- acquiring the Raman spectral image with respect to a spectroscopically imaged sample using the acquisition unit;
- generating spectral difference information that emphasizes a Raman signal corresponding to a protein with respect to a Raman signal corresponding to fat contained in the sample from the Raman spectral image using the generation unit;
- acquiring cell identification information that identifies a cell region in a field of view of the Raman spectral image using the cell region identification unit;
- and
- determining a state of the cell based on the spectral difference information and the cell identification information using the determination unit.
Dependencies are inferred from the claim text.