Bio chip for cell analysis and cell analysis device using same
AssigneePaxwell Co.,Ltd.
InventorDae Hee YI
Life of this patent
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Abstract
A bio-chip for cell analysis according to the present disclosure includes: a sample chamber configured to hold a sample; a first pump connected to one side of the sample chamber and configured to generate air pressure for discharging the sample in the sample chamber; a hydrodynamic coupling region connected to the sample chamber and a sheath-fluid supply channel through which the sheath fluid is supplied, configured so that the sample discharged from the sample chamber and the sheath fluid supplied through the sheath-fluid supply channel are mixed; and a bio-chip outlet channel configured so that the mixed sample and the sheath fluid are discharged from the hydrodynamic coupling region.
Claims17
A bio-chip for cell analysis, comprising:
- a sample chamber configured to hold a sample;
- a first pump connected to one side of the sample chamber and configured to generate air pressure for discharging the sample in the sample chamber;
- a hydrodynamic coupling region connected to the sample chamber and a sheath-fluid supply channel through which sheath fluid is supplied, configured so that the sample discharged from the sample chamber and the sheath fluid supplied through the sheath-fluid supply channel are mixed;
- and
- a bio-chip outlet channel configured so that the mixed sample and sheath fluid are discharged from the hydrodynamic coupling region.
A cell analysis method using a bio-chip, comprising:
- supplying a sample to a sample chamber;
- supplying a sheath fluid to a sheath-fluid chamber;
- discharging the sample in the sample chamber to a hydrodynamic coupling region by a first pump;
- discharging the sheath fluid in the sheath-fluid chamber to the hydrodynamic coupling region by a second pump;
- discharging the mixed sample and the sheath fluid from the hydrodynamic coupling region by a bio-chip outlet channel;
- and
- detecting the number of cells or the cell status in at least one of the sample chamber, the hydrodynamic coupling region, and the bio-chip outlet channel by a laser light source and a photo sensor.
A cell analysis device using a bio-chip, comprising:
- a bio-chip including a sample chamber configured to hold a sample;
- a hydrodynamic coupling region connected to the sample chamber and a sheath-fluid supply channel, configured so that the sample discharged from an outlet of the sample chamber and the sheath fluid supplied through the sheath-fluid supply channel are mixed;
- and
- a bio-chip outlet channel configured so that the mixed sample and the sheath fluid in the hydrodynamic coupling region are discharged;
- one or more laser light sources configured to irradiate laser light from the side of the bio-chip toward at least one of the sample chamber, the hydrodynamic coupling region, and the bio-chip outlet channel;
- and
- one or more photo sensors configured to capture scattered light generated from at least one of the sample chamber, the hydrodynamic coupling region, and the bio-chip outlet channel by irradiation of the laser light.
A cell analysis method using a bio-chip, comprising:
- preparing a bio-chip that includes a sample chamber configured to hold a sample, a sheath-fluid chamber configured to hold the sheath fluid, a hydrodynamic coupling region connected to both the sample chamber and the sheath-fluid chamber so that the sample and the sheath fluid discharged from the sample chamber and the sheath-fluid chamber, respectively, are mixed, and a bio-chip outlet channel configured so that the sample and the sheath fluid mixed in the hydrodynamic coupling region are discharged;
- irradiating laser light from the side of the bio-chip toward at least one of the sample chamber, the hydrodynamic coupling region, and the bio-chip outlet channel by one or more laser light sources;
- and
- capturing scattered light generated from at least one of the sample chamber, the hydrodynamic coupling region, and the bio-chip outlet channel due to the irradiation by the one or more laser light sources by one or more photo sensors.
Dependencies are inferred from the claim text.