Method for detecting the presence, identification and quantification in a blood sample of anticoagulants which are blood coagulation enzymes inhibitors, and means for the implementation thereof

An in vitro method for detecting, in a biological sample, the presence of an inhibitor of a blood coagulation enzyme selected, independently, from factor Xa (FXa) and factor IIa (FIIa), the method comprising the following steps:

• a. making one or more competitive kinetics measurement(s) by carrying out a competitive enzymatic assay on a blood sample which has been previously obtained from a subject, said assay being suitable for carrying out competitive kinetic measurements, either having regard to an inhibitor of factor Xa, or to an inhibitor of factor IIa, then
• b. inputting the kinetic measurement(s) obtained in step a. to a classification decision model A obtained by training an automated supervised learning model, then
• i. if the decision model A excludes the presence of an inhibitor of the blood coagulation enzyme whose presence is sought in the analysed sample, concluding to the absence of said inhibitor, or
• ii. if the decision model A confirms the presence of an inhibitor of the blood coagulation enzyme whose presence is sought in the analysed sample, concluding to the presence of said inhibitor.