Engineered genetic incompatibility in plants
OwnerMichael J. Smanski, Matthew H. Zinselmeier +1inventors
InventorsMichael J. Smanski, Matthew H. Zinselmeier, Juan Armando Casas Mollano
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Abstract
In one aspect, a transgenic plant cell includes a biocontainment system that includes: a coding region whose overexpression alters growth of the cell or impairs cell division; a transcription regulatory region operably linked upstream of the coding region, and including a mutation; and a polynucleotide that encodes a programmable transcription activator engineered to bind to the transcription regulatory region in the absence of the mutation after sexual reproduction, thereby overexpressing the coding region in the absence of the mutation, but does not initiate overexpression of the coding region when the transcription regulatory region includes the mutation.
Claims20
A transgenic plant cell, the cell comprising a biocontainment system that comprises:
- a coding region whose overexpression alters growth of the cell or impairs cell division;
- a transcription regulatory region operably linked upstream of the coding region, and comprising a mutation;
- and
- a polynucleotide that encodes a programmable transcription activator engineered to bind to the transcription regulatory region in the absence of the mutation after sexual reproduction, thereby overexpressing the coding region in the absence of the mutation, but does not initiate overexpression of the coding region when the transcription regulatory region comprises the mutation.
A method of limiting hybridization of a genetically-modified plant with a genetically dissimilar variant, the method comprising:
- providing a plant genetically modified to comprise the biocontainment system of any preceding claim, wherein a cross between the genetically-modified plant and the genetically dissimilar variant plant results in progeny that exhibit a phenotype that is distinct from the genetically-modified plant.
Dependencies are inferred from the claim text.