Production of leghemoglobin in plants
AssigneePioneer Hi-Bred International, Inc.
InventorsJOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU, KNUT MEYER, KEVIN G RIPP
Life of this patent
Estimating the term…
Abstract
Plants and plant tissue such as leaves comprising leghemoglobin are produced by modifying the genome of the plant or introducing leghemoglobin coding or regulatory sequences into the plant. Plants, leaves, fruits, tubers, roots, seeds, and protein compositions comprising leghemoglobin are provided. Protein compositions comprising leghemoglobin, such as isolates and concentrates, can be made from the plants, tissue, fruits, tubers, roots, seeds and leaves. Additionally, methods for generating and using plants, tissue, fruits, tubers, roots, seeds, leaves and protein compositions comprising leghemoglobin are disclosed.
Claims30
A plant leaf comprising a genomic modification, the genomic modification comprising one or more of (i) an insertion of a leghemoglobin coding sequence into a native non-leghemoglobin gene such that the leghemoglobin coding sequence replaces all or part of the native non-leghemoglobin gene coding sequence, (ii) an insertion of a heterologous regulatory enhancer or heterologous promotor sequence operably linked to a native leghemoglobin coding sequence, and (iii) a substitution or deletion wherein the substitution or deletion creates or enhances a regulatory enhancer or promotor sequence of a native leghemoglobin coding sequence, wherein a leghemoglobin protein is expressed in the plant leaf.
-7. (canceled)
-11. (canceled)
A plant leaf comprising a leghemoglobin protein in an amount of at least 0.1% of total protein in the plant leaf, wherein the plant leaf comprises a leghemoglobin coding sequence operably linked to a heterologous regulatory element.
-18. (canceled)
The plant leaf of claim 18, wherein the plant leaf genome has been modified to introduce (i) an insertion of a heterologous regulatory enhancer or heterologous promoter sequence operably linked to the native leghemoglobin coding sequence, or (ii) a substitution wherein the substitution creates or enhances a native leghemoglobin coding sequence regulatory enhancer or a native leghemoglobin coding sequence promotor sequence.
21-22. (canceled)
(canceled)
-27. (canceled)
-31. (canceled)
-44. (canceled)
(canceled)
-74. (canceled)
Dependencies are inferred from the claim text.