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US 2026/0070055 A1Published application
Methods and compositions for surface functionalization of optical semiconductor-integrated biochips
AssigneeInSilixa, Inc.
InventorsAndrea Cuppoletti, Arjang Hassibi, Lei Pei, Yang Liu, Kshama Jirage, Arun Manickam
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Abstract
The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.
Claims21
-30. (canceled)
A method of immobilizing a nucleic acid construct on a substrate surface with controlled density, comprising:
- on the substrate surface, depositing a first silane comprising a first reactive group which is an azide or alkyne, and a second silane lacking said first reactive group and not capable of reacting with the nucleic acid construct;
- contacting the substrate surface with the nucleic acid construct, wherein the nucleic acid construct comprises a second reactive group capable of reacting with the first reactive group via a strain-promoted alkyne-azide cycloaddition (SPAAC) reaction, thereby immobilizing the nucleic acid construct on the surface of the substrate.
Dependencies are inferred from the claim text.