Composition of chromatographic sorbents and methods for analysis of antibody drug conjugates

Abstract

The present disclosure provides chromatography compositions and methods for separation of biomolecules, in particular, stationary phases and chromatography columns for improved separation and resolution of DAR species in antibody drug conjugates (ADCs). In one example, a method comprises: (a) exposing the sample to a chromatographic stationary phase of a reverse phase chromatography column, and (b) applying an eluent to the chromatography column to elute the DAR species of antibody-drug conjugate in their native state, wherein the chromatographic stationary phase comprises: a solid support; and a polymer coating on a surface of the solid support; wherein the polymer coating comprises a copolymer comprising a first hydrophilic monomer and a second hydrophilic monomer, wherein the first hydrophilic monomer and second hydrophilic monomer have different hydrophilicity, wherein the copolymer has a tuned hydrophilicity determined by a molar ratio of the first hydrophilic monomer to the second hydrophilic monomer.

Dependencies are inferred from the claim text.